"Wherein, YPEG-NHS can be prepared as described in EP1496076.
"The reaction of G-CSF with YPEG to form YPEG-G-CSF is shown as below:
"The reaction is performed under mild reaction conditions, pH 6.0-10, preferably 8.0, temperature 0-20.degree. C., mixed by stirring etc. The modification site of pegylation is determined by performing molecular weight analysis on YPEG-G-CSF combined with molecular fingerprint mapping and trypsin digestion pattern and N-terminal sequencing of the protein. The inventor found that the pegylation site on G-CSF was on lysine of position 17, and the product of the modification was recorded as YPEG-G-CSF (17).
"The separation and purification of YPEG-G-CSF (17) can be performed by methods such as ion exchange. In one embodiment, the prepared YPEG-G-CSF is passed through a cationic ion exchange column, the fourth active peak is collected, and the product is further purified by a Sephacryl S-400HR column to obtain the purified YPEG-G-CSF (17).
"The invention also provides the use of the YPEG-G-CSF of the present invention or the composition comprising the YPEG-G-CSF of the present invention in treating disease in need of G-CSF treatment. The clinical use of the pegylated G-CSF or the composition comprising the pegylated G-CSF of the present invention is the same as that of G-CSF, and they all can be applied in treating granulocytopenia-related diseases such as granulocytopenia induced by severe infection, treatment for leukemia, stern cell transplantation, and malignant solid tumor chemotherapy (
URL and more information on this patent, see: Wang, Shiyuan; Zheng, Jianhua; Sun, Li; Cai, Huili; Yang,
Keywords for this news article include: Ions, Alkenes, Polyenes, Cytokines, Treatment, Disulfides, Leukocytes, Amino Acids, Electrolytes, Hydrocarbons, Legal Issues, Glycoproteins, Myeloid Cells, Interferon-alpha, Interferon Type I, Biological Factors, Inorganic Chemicals, Polyethylene Glycols, Hematopoietic Cell Growth Factors.
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