"U.S. Pat. No. 6,730,883, patented
"U.S. Pat. No. 5,552,580, patented
"These attempted solutions were not completely effective since, by definition, the use of a heated lid required extra electronics and components compared to a system without a heated lid.
"Another proposed solution was to overlay mineral oil atop of the liquid reagents to prevent evaporation at the reaction temperature.
"This attempted solution was not completely effective since mineral oil represents an extra reagent that must be added in an extra step. Both of these solutions complicate the process of PCR.
"In addition, real-time thermal cyclers combine a thermal cycling apparatus with a system for optical detection of fluorescence generated by a successful PCR. Some Instruments excite and image the reaction through the top of the tube. For example, this is the setup of the 'Stepfme' system from
"Other systems excite the reaction from the side and image the reaction from the bottom of the tube. For example, this is the setup of the Spartan DX.TM. system from Spartan Bioscience. A problem using such system occurs because standard PCR tubes have curved bottoms. The curvature reflects and refracts both the excitation and emission light. This results in light artifacts that reduce sensitivity and specificity of the optical detection."
In addition to obtaining background information on this patent, NewsRx editors also obtained the inventors' summary information for this patent: "Aims of the Invention
"An object of one aspect of the present Invention is to minimize evaporation of the biological fluid being subjected to the PCR reaction.
"Another object of one aspect of the present invention is to improve optical detection of the biological fluid being subjected to the PCR reaction.
"The invention in its general form will first be described, and then its implementation terms of specific embodiments will be detailed with reference to the drawings following hereafter. These embodiments are intended to demonstrate the principle of the invention, and the manner of its implementation. The invention in its broadest sense and more specific forms will then be further described, and defined, in each of the individual claims which conclude this Specification."
For more information, see this patent: Harder, Chris; Cloake, Martin; Perreault, Michel; Lem, Paul; Shayanpour, Alan. Tube for DNA Reactions. U.S. Patent Number 8528777, filed
Keywords for this news article include: Chemicals, Chemistry,
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