"In some embodiments, X.sub.1, X.sub.2, X.sub.3, X.sub.4, and X.sub.5 each can be deleted or be kept or be replaced by (substituted with) another residue. Preferred substitutions are homologous substitutions, wherein amino acids of similar properties are substituted. These changes can be made when the minimal motifs or the 8-mer peptides containing each minimal motif are prepared by chemical synthesis as single peptides or by expression as peptides, e.g., fused with GST188 or with other vector proteins.
"A synthetic peptide of the invention may contain a suitable length for the intended purpose, such as 5-8 amino acid residues, 6-10 amino acid residues, 8-15 amino acid residues, 10-20 amino acid residues, 10-30 amino acid residues, or the like. In this description, any numeric range is intended to include each individual number within the range as if they are explicitly described. These synthetic peptides may be referred to as 'isolated peptides.' The term 'isolated peptide' is used herein to denote that the peptide is not contained in its nature protein or is not in natural occurrence.
"The epitope minimal motifs in L1 protein from HPV 58 described above can be used to develop preventative and/or therapeutic multi-epitope peptide vaccines or biologics against HPV 58. For example, peptides containing these motifs may be used to immunize individuals or to produce antibodies for diagnostic or therapeutic uses.
"The epitope minimal motifs in L1 protein from HPV 58 described above can be used as marker antigen(s) to develop detective kits for HPV 58. They can be synthesized as a single peptide, which may contain one or more motifs, or can be expressed biologically either as a single peptide fused with a suitable protein (e.g., GST188 or with other vector protein), or as multi-epitope peptide antigens.
"The peptides each containing an epitope minimal motif in the L1 protein of from HPV 58 described above can be used to develop preventative and/or therapeutic multi-epitope peptide vaccines or biologics against HPV 58. The peptides each containing an epiptope minimal motif in the L1 protein of HPV 58 described above can be used as marker antigen(s) to develop detective kits for HPV 58. They can be synthesized as a single peptide or can be expressed biologically either as a single peptide fused with a suitable protein (e.g., GST188 or with other vector protein), or as multi-epitope peptide antigens.
"Of the eighteen epitope motifs in this invention, ten are 100% conservative epitope motifs and one is a highly conservative epitope motif among homologous proteins of HR-HPVs. They can be used as candidate epitope peptides to develop 'universal' preventative and/or therapeutic multi-epitope peptide vaccines against different types of HPVs. These motifs are listed as below:
"HPV58/L1-5.sup.213-217 (PLELF) (expressed as PLELX, X is an alternative amino acid residue)
BRIEF DESCRIPTION OF THE DRAWINGS
"FIG. 1. Mapping of Linear B-Cell Epitopes in L1 Protein from HPV 58 by Western blotting using rabbit serum raised against r-L1N or r-L1C. Lanes 1-24 (A) and lanes 25-60 (C): the 18-mer peptides (fused with GST188) of expressed P1-P60; Lane 61: the 14-mer peptide of positive control. FIGS. 1B and 1D: the mapping results of P1-P60 using pre-immune rabbit serum. Arrows in FIG. 1A, C and D indicated that the reactive 18-mer peptide fused with GST188 and positive control (HPV58/E6-13.sup.73-87 fused with GST188) can be recognized by pre-immune rabbit serum.
"FIG. 2. Mapping the Minimal motif of the reactive epitope peptide P27 (A) and common sequence analysis (B). A: Lanes 1-11: a set of 8-mer peptides (P142-P152) covering the reactive 18-mer peptide P27; B: The figure shows the sequences of 8-mer peptides P142-P52, their position in HPV 58-L1 protein, and a BCE minimal motif identified by Western blotting. The arrows indicated the reactive bands of P142-P145 with Western blotting, respectively; The shaded area indicated the common sequence among the reactive peptides of P142-P145."
For more information, see this patent application: Xu, Wanxiang; Gu, Shaohua; He, Yaping; Ji, Chaoneng; Tang, Haiping; Wang, Jian; Sun, Zhida; Xie, Yi. Minimal Motifs of Linear B-Cell Epitopes in L1 Protein from Human Papillomavirus Type 58 and Their Applications. U.S. Patent Application Serial Number 364952, filed
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