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Researchers from University of Padua Describe Findings in Chromatography (OFFGEL fractionation of peptides: Where really is your sample?)

September 12, 2014

By a News Reporter-Staff News Editor at Science Letter -- A new study on Science is now available. According to news reporting out of Padua, Italy, by NewsRx editors, research stated, "Shotgun proteomics of complex samples is generally coupled with at least one peptide fractionation step and, to this effect, peptide isoelectric focusing (IEF) in immobilized pH gradient (IPG) is one of the most used techniques. Fractionation with the OFFGEL 3100 Agilent Technologies apparatus allows the easy recovery of peptides that, after focusing, diffuse into the liquid phase above the gel strip."

Our news journalists obtained a quote from the research from the University of Padua, "In this work we investigate the efficiency of peptide diffusion during OFFGEL fractionation and demonstrate that a recovery based only on the spontaneous diffusion process is far from being optimal. We show that a simple additional extraction step with acetonitrile increases of about 40% the amount of material that can be recovered after the focusing."

According to the news editors, the research concluded: "Moreover, we show that the two populations of peptides obtained from the passive elution and from the extraction process are also qualitatively different and only partially overlapping."

For more information on this research see: OFFGEL fractionation of peptides: Where really is your sample? Journal of Chromatography A, 2014;1355():278-283. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Journal of Chromatography A -

Our news journalists report that additional information may be obtained by contacting C. Franchin, University of Padua, Dept. of Med, I-35121 Padua, Italy. Additional authors for this research include M. Pivato, M. Rattazzi, G. Arrigoni and R. Millioni (see also Science).

Keywords for this news article include: Padua, Italy, Europe, Science

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Source: Science Letter

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