Study Results from Singapore National University Broaden Understanding of Stem Cells (Evaluation of human embryonic stem cells and their differentiated fibroblastic progenies as cellular models for in vitro genotoxicity screening)
By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Stem Cell Research have been presented. According to news originating from Singapore, Singapore, by NewsRx correspondents, research stated, "This study evaluated human embryonic stem cells (hESC) and their differentiated fibroblastic progenies as cellular models for genotoxicity screening. The DNA damage response of hESCs and their differentiated fibroblastic progenies were compared to a fibroblastic cell line (HEPM, CRL1486) and primary cultures of peripheral blood lymphocytes (PBL), upon exposure to Mitomycin C, gamma irradiation and H2O2."
Our news journalists obtained a quote from the research from Singapore National University, "It was demonstrated that hESC-derived fibroblastic progenies (H1F) displayed significantly higher chromosomal aberrations, micronuclei formation and double strand break (DSB) formation, as compared to undifferentiated hESC upon exposure to genotoxic stress. Nevertheless, H1F cell types displayed comparable sensitivities to genotoxic challenge as HEPM and PBL, both of which are representative of somatic cell types commonly used for genotoxicity screening. Subsequently, transcriptomic and pathways analysis identified differential expression of critical genes involved in cell death and DNA damage response upon exposure to gamma irradiation. The results thus demonstrate that hESC-derived fibroblastic progenies are as sensitive as commonly-used somatic cell types for genotoxicity screening."
According to the news editors, the research concluded: "Moreover, hESCs have additional advantages, such as their genetic normality compared to immortalized cell lines, as well as their amenability to scale-up for producing large, standardized quantities of cells for genotoxicity screening on an industrial scale, something which can never be achieved with primary cell cultures."
For more information on this research see: Evaluation of human embryonic stem cells and their differentiated fibroblastic progenies as cellular models for in vitro genotoxicity screening. Journal of Biotechnology, 2014;184():154-168. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Biotechnology - www.elsevier.com/wps/product/cws_home/505515)
The news correspondents report that additional information may be obtained from K.J. Vinoth, Singapore National University, Tembusu College, Singapore 117548, Singapore. Additional authors for this research include J. Manikandan, S. Sethu, L. Balakrishnan, A. Heng, K. Lu, M.P. Hande and T. Cao (see also Stem Cell Research).
Keywords for this news article include: Singapore, Singapore, Asia, Connective Tissue Cells, Fibroblasts, Stem Cell Research
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