Study Findings on DNA Research Are Outlined in Reports from Tsinghua University (Multiresponsive Rolling Circle Amplification for DNA Logic Gates Mediated by Endonuclease)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in DNA Research. According to news reporting from Beijing, People's Republic of China, by NewsRx journalists, research stated, "Rolling circle amplification (RCA), an efficient isothermal amplification method allowing the polymerase-mediated generation of long single-stranded DNA molecules made of tandem repeats, has been widely used in biomedical and nanotechnology fields due to structural and compositional versatility of its components. In this work, we confer multiresponsiveness to RCA reactions by designing dumbbell-shaped DNA templates and hairpin probes containing different endonuclease cleavage sites."
The news correspondents obtained a quote from the research from Tsinghua University, "Endonucleases trigger the release of RCA primers or the cleavage of DNA templates, which controls subsequent RCA reactions. A set of one-input and two-input DNA logic gates, which use endonucleases or hairpin probes as inputs, including YES, NOT, AND, OR, NOR, and INHIBIT, are constructed on the basis of our proposed multiresponsive RCA reactions. We demonstrate flexibility and scalability of these logic gates by integrating them to fabricate more complex three-input logic circuits (AND-OR and NOR-AND circuits). Moreover, our strategy is used to construct an assay system for endonuclease activity."
According to the news reporters, the research concluded: "Our proposed method might be applicable in the multichannel detection of endonucleases, nucleic adds, and other biomolecules."
For more information on this research see: Multiresponsive Rolling Circle Amplification for DNA Logic Gates Mediated by Endonuclease. Analytical Chemistry, 2014;86(15):7813-7818. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting W.D. Xu, Tsinghua Univ, Dept. of Chem, Beijing Key Lab Analyt Methods & Instrumentat, Beijing 100084, People's Republic of China. Additional authors for this research include R.J. Deng, L.D. Wang and J.H. Li (see also DNA Research).
Keywords for this news article include: Beijing, People's Republic of China, Asia, DNA Research
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