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Study Findings from C.L. Phillips et al Broaden Understanding of Cultured Cells [The response effect of pheochromocytoma (PC12) cell lines to...

September 12, 2014



Study Findings from C.L. Phillips et al Broaden Understanding of Cultured Cells [The response effect of pheochromocytoma (PC12) cell lines to oxidized multi-walled carbon nanotubes (o-MWCMTs)]

By a News Reporter-Staff News Editor at Health & Medicine Week -- Current study results on Cultured Cells have been published. According to news originating from Wits, South Africa, by NewsRx correspondents, research stated, "The applications of oxidized carbon nanotubes (o-CNTs) have shown potentials in novel drug delivery including the brain which is usually a challenge. This underscores the importance to study its potential toxic effect in animals."

Our news journalists obtained a quote from the research, "Despite being a promising tool for biomedical applications little is known about the safety of drugs in treating brain diseases. The toxicity of oxidized multi-walled carbon nanotubes (o-MWCNTs) are of utmost concern and in most in-vitro studies conducted so far are on dendritic cell (DC) lines with limited data on PC12 cell lines. We focused on the effect of o-MWCNTs in PC12 cells in vitro: a common model cell for neurotoxicity. The pristine multi-walled carbon nanotubes (p-MWCNTs) were produced by the swirled floating catalytic chemical vapour deposition method (SFCCVD). The p-MWCNTs were then oxidized using purified H2SO4/HNO3 (3:1v/v) and 30% HNO3 acids to produce o-MWCNTs. The Brunauer-Emmett-Teller (BET), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), thermogravimetric analyser (TGA) and Raman spectroscopy techniques were used to characterize the MWCNTs. The PC12 cells were cultured in RPMI medium containing concentrations of o-MWCNTs ranging from 50 to 200 g/ml. The o-MWCNTs demonstrated slight cytotoxicity at short time period to PC12 neuronal cells whilst at longer time period, no significant (p >0.05) toxicity was observed due to cell recovery."

According to the news editors, the research concluded: "The o-MWCNTs did not affect the growth rate and viability of the PC12 cells due to lack of considerable toxicity in the cells during the observed time period but further investigations are required to determine cell recovery mechanism."

For more information on this research see: The response effect of pheochromocytoma (PC12) cell lines to oxidized multi-walled carbon nanotubes (o-MWCMTs). African Health Sciences, 2013;13(4):947-54 (see also Cultured Cells).

The news correspondents report that additional information may be obtained from C.L. Phillips, School of Chemical and Metallurgical Engineering, University of the Witwatersr andJohannesburg, P, Bag 3, Wits 2050, South Africa. Additional authors for this research include C.S. Yah, S.E. Iyuke, V. Pillay, K. Rumbold and Y. Choonara.

Keywords for this news article include: Wits, South Africa, Africa, Carbon Nanotubes, Cell Line, Cultured Cells, Fullerenes.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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