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Studies from Merck & Company Update Current Data on Biotechnology (Microscale acoustic disruption of mammalian cells for intracellular product...

September 10, 2014

Studies from Merck & Company Update Current Data on Biotechnology (Microscale acoustic disruption of mammalian cells for intracellular product release)

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Technology have been published. According to news reporting from West Point, Pennsylvania, by NewsRx journalists, research stated, "High-throughput microscale models for cell culture are critical for biopharmaceutical process development and drug discovery compound screening. While analytical methods are readily available for quantifying cell number and secreted product concentration, the recovery and measurement of intracellular products are significantly affected by the method of cell disruption."

The news correspondents obtained a quote from the research from Merck & Company, "For example, the detergents often used in product extraction are incompatible with lipid-enveloped viruses. To provide an alternative to detergent-mediated disruption of mammalian cells, we have developed an effective yet gentle mechanical method compatible with 96-well plates using adaptive focused acoustics technology. This method was adapted for the release of Varicella-Zoster virus from MRC-5 cells and then applied to investigate infectious virus yield as a function of the cell density at infection."

According to the news reporters, the research concluded: "This microscale, high-throughput mechanical cell disruption method may be applicable to a variety of mammalian cell culture systems and intracellular products, thus expanding the scope of high-throughput screening."

For more information on this research see: Microscale acoustic disruption of mammalian cells for intracellular product release. Journal of Biotechnology, 2014;184():146-153. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Journal of Biotechnology -

Our news journalists report that additional information may be obtained by contacting M. Ly, Merck & Co Inc, Vaccine R&D, West Point, PA 19486, United States. Additional authors for this research include F. Lu, G. Maheshwari and S. Subramanian (see also Technology).

Keywords for this news article include: West Point, Pennsylvania, United States, North and Central America, Technology

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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