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Studies from Institute of Cancer Further Understanding of Engineering (Lentiviral protein delivery of meganucleases in human cells mediates gene...

September 11, 2014



Studies from Institute of Cancer Further Understanding of Engineering (Lentiviral protein delivery of meganucleases in human cells mediates gene targeting and alleviates toxicity)

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators discuss new findings in Engineering. According to news reporting from London, United Kingdom, by NewsRx journalists, research stated, "Site-specific endonucleases can be engineered for custom recognition of any genetic locus and used for gene targeting. Yet, the prolonged expression and accumulation of these nucleases in cells lead to toxic effect."

The news correspondents obtained a quote from the research from the Institute of Cancer, "Here we describe an efficient and quantitative method for introducing nucleases into cells as proteins packaged within lentiviral vector particles. I-Crel-derived meganucleases, which can be engineered as single-chain proteins, were incorporated into lentiviral vector particles either without modification or as fusions with cyclophilin A. The small amount of nuclease delivered by the viral particles is sufficient to induce efficient targeted mutagenesis in human HEK293H and primary T cells."

According to the news reporters, the research concluded: "When a repair template sequence was packaged in the lentiviral vector, high levels of homologous gene targeting were obtained and toxicity was markedly reduced."

For more information on this research see: Lentiviral protein delivery of meganucleases in human cells mediates gene targeting and alleviates toxicity. Gene Therapy, 2014;21(8):759-766. Gene Therapy can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group - www.nature.com/; Gene Therapy - www.nature.com/gt/)

Our news journalists report that additional information may be obtained by contacting C. He, UCL, Inst Canc, London, United Kingdom. Additional authors for this research include A. Gouble, A. Bourdel, V. Manchev, L. Poirot, F. Paques, P. Duchateau, A. Edelman and O. Danos (see also Engineering).

Keywords for this news article include: London, United Kingdom, Europe, Engineering

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Gene Therapy Weekly


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