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Studies from Cancer Hospital Provide New Data on DNA Research (Proximity Hybridization Regulated DNA Biogate for Sensitive Electrochemical...

September 9, 2014

Studies from Cancer Hospital Provide New Data on DNA Research (Proximity Hybridization Regulated DNA Biogate for Sensitive Electrochemical Immunoassay)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting from Nanjing, People's Republic of China, by NewsRx journalists, research stated, "An electrochemical DNA biogate was designed for highly sensitive homogeneous electrochemical immunoassay by combining target-induced proximity hybridization with a mesoporous silica nanoprobe (MSN). The electroactive methylene blue (MB) was sealed in the inner pores of MSN with single-stranded DNA."

The news correspondents obtained a quote from the research from Cancer Hospital, "In the presence of target protein and two DNA-labeled antibodies, the formed proximate complex could hybridize with the DNA strand to form a rigid double-stranded structure and thus open the biogate, which led to the release of MB entrapped in the MSN. The target protein-dependent amount of released MB could be conveniently monitored with a screen-printed carbon electrode. Moreover, the detachment process of MB could be further amplified with an in situ enzymatic recycling binding of the proximate complex with the single-stranded DNA. Using prostate-specific antigen as a model target, the proposed assay showed a wide detection range from 0.002 to 100 ng mL(-1) with a detection limit of 1.3 pg mL(-1). This strategy was simple and universal for various analytes with different affinity ligands."

According to the news reporters, the research concluded: "This method possessed great potential for convenient point-of-care testing and commercial application."

For more information on this research see: Proximity Hybridization Regulated DNA Biogate for Sensitive Electrochemical Immunoassay. Analytical Chemistry, 2014;86(15):7494-7499. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Analytical Chemistry -

Our news journalists report that additional information may be obtained by contacting K.W. Ren, Jiangsu Canc Hosp, Nanjing 210009, People's Republic of China. Additional authors for this research include J. Wu, Y. Zhang, F. Yan and H.X. Ju (see also DNA Research).

Keywords for this news article include: Nanjing, People's Republic of China, Asia, Chemicals, Chemistry, DNA Research, Electrochemical

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Life Science Weekly

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