Studies Conducted at University of Mississippi on Escherichia Coli Recently Reported (Novel bacterial bioassay for a high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors)
By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Proteobacteria have been published. According to news originating from Oxford, Mississippi, by NewsRx correspondents, research stated, "Plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of synthetic beta-triketone herbicides that are currently used commercially. Their mode of action is based on an irreversible inhibition of HPPD."
Our news journalists obtained a quote from the research from the University of Mississippi, "Therefore, this inhibitory capacity was used to develop a whole-cell colorimetric bioassay with a recombinant Escherichia coli expressing a plant HPPD for the herbicide analysis of beta-triketones. The principle of the bioassay is based on the ability of the recombinant E. coli clone to produce a soluble melanin-like pigment, from tyrosine catabolism through p-hydroxyphenylpyruvate and homogentisate. The addition of sulcotrione, a HPPD inhibitor, decreased the pigment production. With the aim to optimize the assay, the E. coli recombinant clone was immobilized in sol-gel or agarose matrix in a 96-well microplate format. The limit of detection for mesotrione, tembotrione, sulcotrione, and leptospermone was 0.069, 0.051, 0.038, and 20 mu M, respectively, allowing to validate the whole-cell colorimetric bioassay as a simple and cost-effective alternative tool for laboratory use."
According to the news editors, the research concluded: "The bioassay results from sulcotrione-spiked soil samples were confirmed with high-performance liquid chromatography."
For more information on this research see: Novel bacterial bioassay for a high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors. Applied Microbiology and Biotechnology, 2014;98(16):7243-7252. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)
The news correspondents report that additional information may be obtained from E. Rocaboy-Faquet, University of Mississippi, Nat Prod Utilizat Res Unit, Dept. of Agriculture, ARS, Oxford, MS 38677, United States. Additional authors for this research include T. Noguer, S. Romdhane, C. Bertrand, F.E. Dayan and L. Barthelmebs (see also Proteobacteria).
Keywords for this news article include: Oxford, Mississippi, United States, North and Central America, 4-Hydroxyphenylpyruvate Dioxygenase, Dioxygenases, Enterobacteriaceae, Enzymes and Coenzymes, Escherichia, Gammaproteobacteria, Oxidoreductases
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