Studies Conducted at Chinese Academy of Sciences on Proton Pumps Recently Reported (Highly Sensitive Real-Time Assay of Inorganic Pyrophosphatase Activity Based on the Fluorescent Gold Nanoclusters)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Carrier Proteins have been published. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "On the basis of the competition assay approach and fluorescent 11-mercaptoundecanoic acid-capped AuNCs (AuNCs@11-MUA) with unique optical properties, a convenient, reliable and highly sensitive real-time assay of pyrophosphatase (PPase) activity is established and developed for the first time. Pyrophosphate (PP could recover the Cu2+-quenched AuNC5@11-MUA fluorescence selectively owing to the higher binding affinity between PPi and Cu2+ than that between 11-MUA and Cu2+."
Our news editors obtained a quote from the research from the Chinese Academy of Sciences, "Whereas PPase could catalyze the hydrolysis of PPi, thus released Cu2+, leading to fluorescence requenching of the AuNCs@11-MUA. In the assay, a good linearity between the fluorescence response and PPase activity within a range from 1 to 20 mU is found, with a detection limit of less than 1 mU, which is better than other PPase assays using PPi as the substrate. Additionally, we demonstrate that our AuNCs@11-MUA-based fluorescent assay can be applied to assay the PPase activity in real biological samples such as the cell lysate."
According to the news editors, the research concluded: "This strategy paves a new avenue for exploring the sensing applications of fluorescence AuNCs and improving the development of competition assay approach."
For more information on this research see: Highly Sensitive Real-Time Assay of Inorganic Pyrophosphatase Activity Based on the Fluorescent Gold Nanoclusters. Analytical Chemistry, 2014;86(15):7883-7889. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news editors report that additional information may be obtained by contacting J. Sun, Univ Chinese Academy Sci, Beijing 100039, People's Republic of China. Additional authors for this research include F. Yang, D. Zhao and X.R. Yang (see also Carrier Proteins).
Keywords for this news article include: Beijing, People's Republic of China, Asia, Carrier Proteins, Emerging Technologies, Gold Nanoclusters, Inorganic Pyrophosphatase, Ion Pumps, Membrane Proteins, Membrane Transport Proteins, Nanotechnology, Proton Pumps
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