Researchers from University of California Report Details of New Studies and Findings in the Area of Economic Research (Limited Dissemination and Shedding of the UL128 Complex-Intact, UL/b '-Defective Rhesus Cytomegalovirus Strain 180.92)
By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Herpesviridae Infections are discussed in a new report. According to news reporting originating from Davis, California, by NewsRx correspondents, research stated, "The UL128 complex of human cytomegalovirus (CMV) is a major determinant of viral entry into epithelial and endothelial cells and a target for vaccine development. The UL/b' region of rhesus CMV contains several open reading frames, including orthologs of the UL128 complex."
Our news editors obtained a quote from the research from the University of California, "We recently showed that the coding content of the rhesus CMV (RhCMV) UL/b' region predicts acute endothelial tropism and long-term shedding in vivo in the rhesus macaque model of CMV infection. The laboratory-passaged RhCMV 180.92 strain has a truncated UL/b' region but an intact UL128 complex. To investigate whether the presence of the UL128 complex alone was sufficient to confer endothelial and epithelial tropism in vivo, we investigated tissue dissemination and viral excretion following experimental RhCMV 180.92 inoculation of RhCMV-seronegative rhesus macaques. We show the presence of at least two virus variants in the RhCMV 180.92 infectious virus stock. A rare variant noted for a nontruncated wild-type-virus-like UL/b' region, rapidly emerged during in vivo replication and showed high-level replication in blood and tissues and excretion in urine and saliva, features similar to those previously reported in naturally occurring wild-type RhCMV infection. In contrast, the predominant truncated version of RhCMV 180.92 showed significantly lower plasma DNAemia and limited tissue dissemination and viral shedding."
According to the news editors, the research concluded: "These data demonstrate that the truncated RhCMV 180.92 variant is attenuated in vivo and suggest that additional UL/b' genes, besides the UL128 complex, are required for optimal in vivo CMV replication and dissemination."
For more information on this research see: Limited Dissemination and Shedding of the UL128 Complex-Intact, UL/b '-Defective Rhesus Cytomegalovirus Strain 180.92. Journal of Virology, 2014;88(16):9310-9320. Journal of Virology can be contacted at: Amer Soc Microbiology, 1752 N St NW, Washington, DC 20036-2904, USA. (American Society for Microbiology - www.asm.org; Journal of Virology - jvi.asm.org)
The news editors report that additional information may be obtained by contacting B.T. Assaf, University of California, Calif Natl Primate Res Center, Davis, CA 95616, United States. Additional authors for this research include K.G. Mansfield, L. Strelow, S.V. Westmoreland, P.A. Barry and A. Kaur (see also Economic Research).
Keywords for this news article include: Davis, California, United States, North and Central America, Betaherpesvirinae, CMV, Cytomegalovirus Infections, Cytomegalovirus Vaccines, DNA Virus Infections, DNA Viruses, Genetics, Herpesviridae Infections, Viral Vaccines, Virology
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC