Researchers from University of Alberta Report Recent Findings in DNA Research (Sequential strand displacement beacon for detection of DNA coverage on functionalized gold nanoparticles)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on DNA Research is now available. According to news reporting out of Edmonton, Canada, by NewsRx editors, research stated, "Functionalizing nanomaterials for diverse analytical, biomedical, and therapeutic applications requires determination of surface coverage (or density) of DNA on nanomaterials. We describe a sequential strand displacement beacon assay that is able to quantify specific DNA sequences conjugated or coconjugated onto gold nanoparticles (AuNPs)."
Our news journalists obtained a quote from the research from the University of Alberta, "Unlike the conventional fluorescence assay that requires the target DNA to be fluorescently labeled, the sequential strand displacement beacon method is able to quantify multiple unlabeled DNA oligonucleotides using a single (universal) strand displacement beacon. This unique feature is achieved by introducing two short unlabeled DNA probes for each specific DNA sequence and by performing sequential DNA strand displacement reactions. Varying the relative amounts of the specific DNA sequences and spacing DNA sequences during their coconjugation onto AuNPs results in different densities of the specific DNA on AuNP, ranging from 90 to 230 DNA molecules per AuNP. Results obtained from our sequential strand displacement beacon assay are consistent with those obtained from the conventional fluorescence assays. However, labeling of DNA with some fluorescent dyes, e.g., tetramethylrhodamine, alters DNA density on AuNP. The strand displacement strategy overcomes this problem by obviating direct labeling of the target DNA."
According to the news editors, the research concluded: "This method has broad potential to facilitate more efficient design and characterization of novel multifunctional materials for diverse applications."
For more information on this research see: Sequential strand displacement beacon for detection of DNA coverage on functionalized gold nanoparticles. Analytical Chemistry, 2014;86(12):6138-43. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting R.E. Paliwoda, Dept. of Chemistry and Dept. of Laboratory Medicine and Pathology, Division of Analytical and Environmental Toxicology, University of Alberta , Edmonton, Alberta T6G2G3, Canada. Additional authors for this research include F. Li, M.S. Reid, Y. Lin and X.C Le (see also DNA Research).
Keywords for this news article include: Edmonton, Alberta, Canada, North and Central America, DNA Research, Emerging Technologies, Nanoparticle, Nanotechnology.
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