Researchers from Qingdao University of Science and Technology Report Findings in Esterases (Sensitive electrochemical assay for T4 polynucleotide kinase activity based on dual-signaling amplification coupled with exonuclease reaction)
By a News Reporter-Staff News Editor at Biotech Week -- A new study on Enzymes and Coenzymes is now available. According to news reporting out of Qingdao, People's Republic of China, by NewsRx editors, research stated, "A novel electrochemical strategy for simple and accurate monitoring of the activity and the inhibition of T4 polynucleotide kinase (PNK) is developed by coupling the lambda exonuclease (lambda exo) cleavage reaction with a dual-signaling amplification method. In the presence of PNK and adenosine triphosphate, the 5'-hydroxyl group of the substrate DNA is phosphorylated, initiating the hydrolysis of the 5'-phosphoryl termini catalyzed by lambda exo, and by combining the resulting G-quadruplex-hemin signal-on probe and the ferrocene signal-off probe, a dual-signaling amplification strategy is realized."
Our news journalists obtained a quote from the research from the Qingdao University of Science and Technology, "This approach exhibits a low detection limit of 0.02 U/mL for PNK activity analysis, which is lower than or comparable to that of the previously reported PNK assays. Additionally, the inhibition effects of two known PNK inhibitors, namely ammonium sulfate and sodium hydrogen phosphate, have also been successfully detected. This new dual-signaling electrochemical biosensor provides a facile platform for the detection of the activity and inhibition of nucleotide kinases."
According to the news editors, the research concluded: "With further exploration, it is possible to extend the as-proposed electrochemical biosensor to the detection of other analytes."
For more information on this research see: Sensitive electrochemical assay for T4 polynucleotide kinase activity based on dual-signaling amplification coupled with exonuclease reaction. Sensors and Actuators B-Chemical, 2014;202():588-593. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Enzymes and Coenzymes).
Our news journalists report that additional information may be obtained by contacting T. Hou, Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao 266042, People's Republic of China. Additional authors for this research include X.Z. Wang, X.L. Liu, C. Pan and F. Li.
Keywords for this news article include: Qingdao, People's Republic of China, Asia, Bioengineering, Bionanotechnology, Biosensing, Biotechnology, Chemicals, Chemistry, Electrochemical, Enzymes and Coenzymes, Esterases, Exonucleases, Kinase, Nanobiotechnology, Nanotechnology
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