Researchers at Ocean University of China Have Reported New Data on Enzymes and Coenzymes (A novel whole genome amplification method using type IIS restriction enzymes to create overhangs with random sequences)
By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting originating from Qingdao, People's Republic of China, by NewsRx correspondents, research stated, "Ligation-mediated polymerase chain reaction (LM-PCR) is a whole genome amplification (WGA) method, for which genomic DNA is cleaved into numerous fragments and then all of the fragments are amplified by PCR after attaching a universal end sequence. However, the self-ligation of these fragments could happen and may cause biased amplification and restriction of its application."
Our news editors obtained a quote from the research from the Ocean University of China, "To decrease the self-ligation probability, here we use type IIS restriction enzymes to digest genomic DNA into fragments with 4-5 nt long overhangs with random sequences. After ligation to an adapter with random end sequences to above fragments, PCR is carried out and almost all present DNA sequences are amplified. In this study, whole genome of Vibrio parahaemolyticus was amplified and the amplification efficiency was evaluated by quantitative PCR."
According to the news editors, the research concluded: "The results suggested that our approach could provide sufficient genomic DNA with good quality to meet requirements of various genetic analyses."
For more information on this research see: A novel whole genome amplification method using type IIS restriction enzymes to create overhangs with random sequences. Journal of Biotechnology, 2014;184():1-6. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Biotechnology - www.elsevier.com/wps/product/cws_home/505515)
The news editors report that additional information may be obtained by contacting X.M. Pan, Ocean University of China, Coll Food Sci & Engn, Qingdao 266003, People's Republic of China. Additional authors for this research include B.H. Wan, C.C. Li, Y. Liu, J. Wang, H.J. Mou and X.G. Liang (see also Enzymes and Coenzymes).
Keywords for this news article include: Qingdao, People's Republic of China, Asia, Enzymes and Coenzymes
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