Researchers at Institute of Chemistry Target Diamines [Poly(m-phenylenediamine)-Based Fluorescent Nanoprobe for Ultrasensitive Detection of Matrix Metalloproteinase 2]
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Diamines is now available. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "A novel fluorescence nanoprobe for the detection of matrix metalloproteinase 2 (MMP2) has been developed by engineering the fluorescein isothiocyanate-labeled peptide onto the surface of poly(m-phenylenediamine) (PMPD) nanoparticles through covalent linkage. The nanoprobe itself displays a low background signal due to the effective fluorescence quenching by electron-rich PMPD, but its reaction with MMP2 causes 11-fold fluorescence enhancement."
Our news editors obtained a quote from the research from the Institute of Chemistry, "Compared with similar fluorescence nanosystems for MMP2 assembled through physical adsorption, the as-prepared nanoprobe is significantly more stable and displays a strikingly higher signal-to-background ratio, which leads to a high sensitivity for MMP2 assay, with a detection limit of 32 pM. Most notably, the nanoprobe has been successfully applied to determine MMP2 in human serum samples, demonstrating that the MMP2 level in serum from colorectal cancer (CRC) patients is 2 times higher than that from healthy people. Moreover, the nanoprobe has also been used to monitor MMP2 secreted by CRC cells that were grown under normoxic and hypoxic conditions, respectively, and the results show that the cells under hypoxic conditions produce higher level of MMP2 than those under normoxic conditions."
According to the news editors, the research concluded: "Our method is simple and can offer a highly sensitive detection of MMP2 in relevant clinical samples."
For more information on this research see: Poly(m-phenylenediamine)-Based Fluorescent Nanoprobe for Ultrasensitive Detection of Matrix Metalloproteinase 2. Analytical Chemistry, 2014;86(15):7719-7725. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news editors report that additional information may be obtained by contacting Z. Wang, Chinese Academy Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, People's Republic of China. Additional authors for this research include X.H. Li, D. Feng, L.H. Li, W. Shi and H.M. Ma (see also Diamines).
Keywords for this news article include: Beijing, People's Republic of China, Asia, Aniline Compounds, Matrix Metalloproteinase, Phenylenediamines, Proteomics
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