Research Data from Technical University Update Understanding of Neurons (Hybrid voltage sensor imaging of eGFP-F expressing neurons in chicken midbrain slices)
By a News Reporter-Staff News Editor at Pain & Central Nervous System Week -- Current study results on Neurons have been published. According to news reporting from Freising Weihenstephan, Germany, by NewsRx journalists, research stated, "Dendritic computation is essential for understanding information processing in single neurons and brain circuits. Optical methods are suited best to investigate function and biophysical properties of cellular compartments at high spatial and temporal resolution."
The news correspondents obtained a quote from the research from Technical University, "Promising approaches include the use of voltage sensitive dyes, genetically encoded voltage sensors, or hybrid voltage sensors (hVoS) consisting of fluorescent proteins and voltage-dependent quenchers that, so far, are not available in avian neuroscience. New method: We have adapted a hVoS system for a chicken midbrain slice preparation by combining genetically expressed farnesylated eGFP with dipicrylamine (DPA). Depending on the cellular potential, DPA is shifted in the membrane, resulting in quenching of eGFP fluorescence linearly to the membrane potential by Forster resonance electron transfer. In ovo electroporation resulted in labelled neurons throughout the midbrain with a high level of fine structural detail. After application of DPA, we were able to optically record electrically evoked action potentials with high signal-to-noise ratio and high spatio-temporal resolution. Comparison with existing methods: Standard methods available for avian neuroscience such as whole-cell patch clamp yield insufficient data for the analysis of dendritic computation in single neurons. The high spatial and temporal resolution of hVoS data overcomesthis limitation. The results obtained by our method are comparable to hVoS data published for mammals."
According to the news reporters, the research concluded: "With the protocol presented here, it is possible to optically record information processing in single avian neurons at such high spatial and temporal resolution, that cellular and subcellular events can be analysed."
For more information on this research see: Hybrid voltage sensor imaging of eGFP-F expressing neurons in chicken midbrain slices. Journal of Neuroscience Methods, 2014;233():28-33. Journal of Neuroscience Methods can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Neuroscience Methods - www.elsevier.com/wps/product/cws_home/506079)
Our news journalists report that additional information may be obtained by contacting S. Weigel, Technical University of Munich, Chair Livestock Biotechnol, D-85354 Freising Weihenstephan, Germany. Additional authors for this research include T. Flisikowska, A. Schnieke and H. Luksch (see also Neurons).
Keywords for this news article include: Freising Weihenstephan, Germany, Europe, Cells, Neurons
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