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Research Conducted at Ankang University Has Updated Our Knowledge about DNA Research (DNA-based hybridization chain reaction for signal amplification...

September 9, 2014



Research Conducted at Ankang University Has Updated Our Knowledge about DNA Research (DNA-based hybridization chain reaction for signal amplification and ultrasensitive chemiluminescence detection of gibberellic acid)

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in DNA Research. According to news reporting originating from Ankang, People's Republic of China, by NewsRx correspondents, research stated, "A novel chemiluminescent (CL) immunoassay protocol for the sensitive determination of Gibberellic acid (GA) using DNA-based hybridization chain reaction (HCR) is described. The carboxyl terminated magnetic beads (MBs) were modified with GA antibody and the gold nanoparticles (AuNPs) signal probes were labeled with the GA antibody and DNA initiator strands."

Our news editors obtained a quote from the research from Ankang University, "In the presence of target GA, the sandwiched immunocomplex was formed between the immobilized antibody on the MBs and the signal antibody on the AuNPs. When the two complementary stable species of DNA hairpins, which are G-rich DNA, were added the hybridization events were happened, thereby resulting forming the long nicked double-helix. Numerous double G-rich DNA were formed on the AuNPs, each of which produced a CL signal within the applied CL reaction. The CL signal was obtained via the instantaneous derivatization reaction between a specific CL reagent, 3,4,5-trimethoxyl-phenylglyoxal (TMPG), and the G-rich DNA on immunocomplex. Under optimal conditions, GA can be detected in the concentration ranged from 0.01 ng/mL to 70 ng/mL, and the limit of detection was 0.003 ng/mL. The reproducibility and selectivity of the developed method were also investigated."

According to the news editors, the research concluded: "In addition, the real samples were assayed by using the developed immunoassay, and the recovery was 96.0-102.0%."

For more information on this research see: DNA-based hybridization chain reaction for signal amplification and ultrasensitive chemiluminescence detection of gibberellic acid. Sensors and Actuators B-Chemical, 2014;202():594-599. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also DNA Research).

The news editors report that additional information may be obtained by contacting X. Hun, Ankang Univ, Dept. of Chem & Chem Engn, Ankang 725000, People's Republic of China. Additional authors for this research include Y.Q. Xu and C. Chen.

Keywords for this news article include: Ankang, People's Republic of China, Asia, Antibodies, Blood Proteins, DNA Research, Immunoglobulins, Immunology

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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