Reports Outline Phagocytosis Study Findings from Finnish Institute of Occupational Health (Phagocytosis of nano-sized titanium dioxide triggers changes in protein acetylation)
By a News Reporter-Staff News Editor at Proteomics Weekly -- Investigators publish new report on Phagocytosis. According to news reporting from Helsinki, Finland, by NewsRx journalists, research stated, "Nano-sized titanium dioxide (nTiO(2)) is one of the most produced engineered nanomaterials and therefore carries a high risk for workplace exposure. In several nanosafety studies, exposure to nTiO(2) has been shown to trigger inflammation in mice lung and to cause oxidative stress."
The news correspondents obtained a quote from the research from the Finnish Institute of Occupational Health, "Here, cytoplasmic proteome changes in human monocyte derived macrophages were investigated with two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry to evaluate the adverse cellular effects after exposure to different types of TiO2 nanoparticles (NPs). Both studied TiO2 NPs (rutile TiO2 with or without silica coating) evoked similar proteome alterations. The identified proteins were linked to metabolic homeostasis, cytoskeleton remodeling and oxidative stress. The abundances of chloride intracellular channel protein 1 and cathepsin D changed only after exposure to nTiO(2) as compared to a coarse particle analog. Enrichment analysis revealed that 70% of the proteins with changed intensities contained known acetylation sites, and it was possible to confirm a significant induction of cytoplasmic protein acetylation after nTiO(2) exposure. The course of the events during phagocytosis could account for the observed membrane maintenance, metabolic and cytoskeletal protein expression changes. Lysine acetylation of cytoplasmic proteins in macrophages is emerging as a major cell regulation mechanism after nTiO(2) exposure. Biological significance While the amount of nanosafety research conducted in recent years has been constantly increasing, proteomics has not yet been utilized widely in this field. In addition, reversible protein post-translational modifications (PTMs) such as acetylation and phosphorylation have not been investigated in-depth in nanomaterial exposed cells. Proteome changes observed in nanomaterial exposed macrophages revealed active phagocytosis of the particles and provided new insights into underlying mechanisms of biological responses to nTiO(2) exposures."
According to the news reporters, the research concluded: "Moreover, reversible protein acetylation might be a major cellular regulation event occurring in nanomaterial exposed cells."
For more information on this research see: Phagocytosis of nano-sized titanium dioxide triggers changes in protein acetylation. Journal of Proteomics, 2014;108():469-483. Journal of Proteomics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Proteomics - www.elsevier.com/wps/product/cws_home/713351)
Our news journalists report that additional information may be obtained by contacting J. Sund, Finnish Inst Occupat Hlth, Nanosafety Res Center, FIN-00250 Helsinki, Finland. Additional authors for this research include J. Palomaki, N. Ahonen, K. Savolainen, H. Alenius and A. Puustinen (see also Phagocytosis).
Keywords for this news article include: Helsinki, Finland, Europe, Chemicals, Chemistry, Connective Tissue Cells, Cytoplasm, Immunology, Intracellular Space, Light Metals, Macrophages, Mononuclear Phagocyte System, Myeloid Cells, Phagocytes, Phagocytosis, Proteins, Proteome, Titanium Dioxide
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