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Recent Findings in Porcine Epidemic Diarrhea Virus Described by P.D. Zhao and Colleagues (Development of a multiplex TaqMan probe-based real-time PCR...

September 9, 2014



Recent Findings in Porcine Epidemic Diarrhea Virus Described by P.D. Zhao and Colleagues (Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus)

By a News Reporter-Staff News Editor at China Weekly News -- Current study results on RNA Viruses have been published. According to news reporting originating in Jiangsu, People's Republic of China, by VerticalNews journalists, research stated, "Since October 2010, porcine diarrhea outbreaks have occurred widely, resulting in major losses in suckling piglets in China. A variant porcine epidemic diarrhea virus (PEDV), characterized by base deletion and insertion in the S gene, compared to classical PEDV CV777, was shown to be responsible for this outbreak."

The news reporters obtained a quote from the research, "In this study, a multiplex TagMan probe-based real-time PCR was developed for detecting PEDV and differentiating the variant from classical PEDV, by using two sets of primers and probes based on the S gene of PEDV. The limits of detection of both variant and classical PEDV were 5 x 10(2) DNA copies. Specificity was determined using eight other viral pathogens of swine. Reproducibility was evaluated using standard dilutions, with coefficients of variation China."

According to the news reporters, the research concluded: "The multiplex TagMan probe-based real-time PCR should be a useful tool for quantifying viral load, detecting PEDV, and differentiating variant from classical PEDV."

For more information on this research see: Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus. Journal of Virological Methods, 2014;206():150-155. Journal of Virological Methods can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Virological Methods - www.elsevier.com/wps/product/cws_home/506080)

Our news correspondents report that additional information may be obtained by contacting P.D. Zhao, Jiangsu Entry Exit Inspect & Quarantine Bur, Nanjing 200001, Jiangsu, People's Republic of China. Additional authors for this research include J. Bai, P. Jiang, T.S. Tang, Y.F. Li, C. Tan and X.L. Shi.

Keywords for this news article include: Jiangsu, People's Republic of China, Asia, Asia, China, Coronaviridae, Coronavirus, Nidovirales, Porcine Epidemic Diarrhea Virus, RNA Viruses, Vertebrate Viruses

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: China Weekly News


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