Recent Findings from Pasteur Institute Provides New Insights into DNA Vaccines (Clustered epitopes within a new poly-epitopic HIV-1 DNA vaccine shows immunogenicity in BALB/c mice)
By a News Reporter-Staff News Editor at AIDS Vaccine Week -- Investigators publish new report on Biotechnology. According to news originating from Tehran, Iran, by NewsRx correspondents, research stated, "Despite a huge number of studies towards vaccine development against human immunodeficiency virus-1, no effective vaccine has been approved yet. Thus, new vaccines should be provided with new formulations."
Our news journalists obtained a quote from the research from Pasteur Institute, "Herein, a new DNA vaccine candidate encoding conserved and immunogenic epitopes from HIV-1 antigens of tat, pol, gag and env is designed and constructed. After bioinformatics analyses to find the best epitopes and their tandem, nucleotide sequence corresponding to the designed multiepitope was synthesized and cloned into pcDNA3.1+ vector. Expression of pcDNA3.1-tat/pol/gag/env plasmid was evaluated in HEK293T cells by RT-PCR and western-blotting. Seven groups of BALB/c mice were intramuscularly immunized three times either with 50, 100, 200 A mu g of plasmid in 2-week intervals or with similar doses of insert-free plasmid. Two weeks after the last injection, proliferation of T cells and secretion of IL4 and IFN-gamma cytokines were evaluated using Brdu and ELISA methods, respectively. Results showed the proper expression of the plasmid in protein and mRNA levels. Moreover, the designed multiepitope plasmid was capable of induction of both proliferation responses as well as IFN-gamma and IL-4 cytokine production in a considerable level compared to the control groups."
According to the news editors, the research concluded: "Overall, our primary data warranted further detailed studies on the potency of this vaccine."
For more information on this research see: Clustered epitopes within a new poly-epitopic HIV-1 DNA vaccine shows immunogenicity in BALB/c mice. Molecular Biology Reports, 2014;41(8):5207-5214. Molecular Biology Reports can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Molecular Biology Reports - www.springerlink.com/content/0301-4851/)
The news correspondents report that additional information may be obtained from N. Jafarpour, Pasteur Inst Iran, Dept. of Virol, Tehran 1316943551, Iran. Additional authors for this research include A. Memarnejadian, M.R. Aghasadeghi, F. Kohram, H. Aghababa, N. Khoramabadi and M. Mahdavi (see also Biotechnology).
Keywords for this news article include: Tehran, Iran, Asia, Acquired Immunodeficiency Syndrome, Biological Products, Biotechnology, DNA Research, DNA Vaccines, HIV Infections, HIV/AIDS, Human Immunodeficiency Virus, Immunization, Medical Devices, Primate Lentiviruses, RNA Viruses, Retroviridae, Synthetic Vaccines, Vaccination, Vertebrate Viruses, Viral DNA, Viral Sexually Transmitted Diseases, Virology
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