New Stem Cells Study Results from Sun Yat Sen University Described (Early administration of tumor necrosis factor-alpha antagonist promotes survival of transplanted neural stem cells and axon myelination after spinal cord injury in rats)
By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Stem Cell Research are presented in a new report. According to news reporting out of Guangdong, People's Republic of China, by NewsRx editors, research stated, "Neural stem cell (NSC) transplantation has been reported to be a leading strategy to stimulate neuroplasticity, repair neuronal loss and promote the morphologic and functional recovery of spinal cord injury (SCI). However, massive death of transplanted NSCs is still a problem, which is considered to be related to a series of pro-inflammatory cytokines that induce apoptosis, extensive demyelination and axonal destruction."
Our news journalists obtained a quote from the research from Sun Yat Sen University, "Tumor necrosis factor alpha (TNF-alpha), as one of the major inflammation initiators, contributes to secondary neural cell death. We previously found that the administration of the TNF-alpha antagonist etanercept during the acute phase of SCI can reduce the apoptosis of neurons and oligodendrocytes. To investigate whether etanercept can suppress transplanted NSC apoptosis and promote NSC survival, axon myelination and functional recovery, we tested the combination strategy of the early administration of etanercept and NSC transplantation. First we observed that etanercept suppressed the TNF-alpha expression and apoptosis of transplanted NSCs by Western blot, TUNEL and immunofluorescence staining. The Basso, Beattle and Bresnahan scale and motor-evoked potential were used to evaluate functional recovery. The results suggest significantly better recovery after combination therapy. Further, histopathological alterations were evaluated by hematoxylin and eosin staining and Nissl staining. These procedures showed that the early administration of etanercept improved survival of neurons in the ventral horn, restored neural morphology and produced a smaller cavity area. We observed most abundant NF-positive fibers after the combination treatment, indicating that combination therapy retained and promoted neural regeneration. Finally, the early suppression of TNF-alpha reduced the occurrence of demyelination, and the combination therapy led to more myelinated axons, as shown by electron microscopy."
According to the news editors, the research concluded: "These data suggest that this strategy significantly protected transplanted NSCs via the anti-inflammation and anti-apoptosis effects of etanercept, promoting re-myelination, neural regeneration and locomotor function."
For more information on this research see: Early administration of tumor necrosis factor-alpha antagonist promotes survival of transplanted neural stem cells and axon myelination after spinal cord injury in rats. Brain Research, 2014;1575():87-100. Brain Research can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Brain Research - www.elsevier.com/wps/product/cws_home/622287)
Our news journalists report that additional information may be obtained by contacting W. Le, Sun Yat Sen UniversityAffiliated Hosp 1, Dept. of Joint Surg, Guangzhou 510080, Guangdong, People's Republic of China. Additional authors for this research include F.X. Wei, J.S. Cen, S.N. Ping, Z.Q. Li, N.N. Chen, S.B. Cui, Y. Wan and S.Y. Liu (see also Stem Cell Research).
Keywords for this news article include: Guangdong, People's Republic of China, Asia, Apoptosis, Axons, Blood Proteins, Cell Surface Extensions, Central Nervous System, Combination Therapy, Cytokines, Glycoproteins, Inflammation, Intercellular Signaling Peptides and Proteins, Monokines, Neurons, Spinal Cord, Stem Cell Research, Tumor Necrosis Factor-alpha, Tumor Necrosis Factors
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