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New Findings Reported from Autonomous University Describe Advances in Transcription Factors (Over-expression of Dof-type transcription factor...

September 10, 2014

New Findings Reported from Autonomous University Describe Advances in Transcription Factors (Over-expression of Dof-type transcription factor increases lipid production in Chlamydomonas reinhardtii)

By a News Reporter-Staff News Editor at Biotech Week -- A new study on Proteins is now available. According to news reporting originating in San Luis Potosi, Mexico, by NewsRx journalists, research stated, "The high demand for less polluting, newer, and cheaper fuel resources has increased the search of the most innovative options for the production of the so-called biofuels. Chlamydomonas reinhardtii is a photosynthetic unicellular algae with multiple biotechnological advantages such as easy handling in the laboratory, a simple scale-up to industrial levels, as well as a feasible genetic modification at nuclear and chloroplast levels."

The news reporters obtained a quote from the research from Autonomous University, "Besides, its fatty acids can be used to produce biofuels. Previous studies in plants have found that the over expression of DOF-type transcription factor genes increases the synthesis and the accumulation of total lipids in seeds. In this context, the over-expression of a DOF-type transcription factor in C. reinhardtii was applied as approach to increase the amount of lipids. The results indicate higher amounts (around 2-fold) of total lipids, which are mainly fatty acids, in the genetically C reinhardtii modified strains when compared with the non-genetically modified strain. In order to elucidate the possible function of the introduced Dof-type transcription factor, we performed a transcription profile of 8 genes involved in fatty acid biosynthesis and 6 genes involved in glycerolipid biosynthesis, by quantitative real time (qRT-PCR). Differential expression profile was observed, which can explain the increase in lipid accumulation. However, these strains did not show notable changes in the fatty acid profile."

According to the news reporters, the research concluded: "This work represents an early effort in generating a strategy to increase fatty acids production in C. reinhardtii and their use in biofuel synthesis."

For more information on this research see: Over-expression of Dof-type transcription factor increases lipid production in Chlamydomonas reinhardtii. Journal of Biotechnology, 2014;184():27-38. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Journal of Biotechnology -

Our news correspondents report that additional information may be obtained by contacting A. Ibanez-Salazar, Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Lab Ingn Biomactores, San Luis Potosi 78210, Mexico. Additional authors for this research include S. Rosales-Mendoza, A. Rocha-Uribe, J.I. Ramirez-Alonso, I. Lara-Hernandez, A. Hernandez-Torres, L.M.T. Paz-Maldonado, A.S. Silva-Ramirez, B. Banuelos-Hernandez, J.L. Martinez-Salgado and R.E. Soria-Guerra (see also Proteins).

Keywords for this news article include: San Luis Potosi, Mexico, North and Central America, Bioengineering, Biofuel, Biotechnology, Energy, Oil and Gas, Proteins, Transcription Factors

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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