New DNA Research Findings Reported from University of Kentucky (Engineered nanopore of Phi29 DNA-packaging motor for real-time detection of single colon cancer specific antibody in serum)
By a News Reporter-Staff News Editor at Cancer Weekly -- Current study results on DNA Research have been published. According to news reporting from Lexington, Kentucky, by NewsRx journalists, research stated, "The ingenious design of the bacterial virus phi29 DNA packaging nanomotor with an elegant and elaborate channel has inspired its application for single molecule detection of antigen/antibody interactions. The hub of this bacterial virus nanomotor is a truncated cone-shaped connector consisting of 12 protein subunits."
The news correspondents obtained a quote from the research from the University of Kentucky, "These subunits form a ring with a central 3.6-nm channel acting as a path for dsDNA to enter during packaging and to exit during infection. The connector has been inserted into a lipid bilayer. Herein, we reengineered an Epithelial Cell Adhesion Molecule (EpCAM) peptide into the C-terminal of nanopore as a probe to specifically detect EpCAM antibody (Ab) in nanomolar concentration at the single molecule level. The binding of Abs sequentially to each peptide probe induced stepwise blocks in current. The distinctive current signatures enabled us to analyze the docking and undocking kinetics of Ab-probe interactions and determine the Kd. The signal of EpCAM antibody can be discriminated from the background events in the presence of nonspecific antibody or serum."
According to the news reporters, the research concluded: "Our results demonstrate the feasibility of generating a highly sensitive platform for detecting antibodies at extremely low concentrations in the presence of contaminants."
For more information on this research see: Engineered nanopore of Phi29 DNA-packaging motor for real-time detection of single colon cancer specific antibody in serum. Acs Nano, 2013;7(11):9814-22. (American Chemical Society - www.acs.org; Acs Nano - www.pubs.acs.org/journal/ancac3)
Our news journalists report that additional information may be obtained by contacting S. Wang, Nanobiotechnology Center, Dept. of Pharmaceutical Sciences, College of Pharmacy and Markey Cancer Center, University of Kentucky , Lexington, Kentucky 40536, United States. Additional authors for this research include F. Haque, P.G. Rychahou, B.M. Evers and P. Guo (see also DNA Research).
Keywords for this news article include: Lexington, Kentucky, United States, North and Central America, Cancer, DNA Research, Oncology, Viral DNA.
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