New Data from Southeast University Illuminate Findings in Cancer Gene Therapy (Galactosylated 2-hydroxypropyl methacrylamide-s-3-guanidinopropyl methacrylamide copolymer as a small hairpin RNA carrier for inhibiting human telomerase reverse ...)
By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Current study results on Biotechnology have been published. According to news reporting originating from Jiangsu, People's Republic of China, by NewsRx correspondents, research stated, "In the present study, a well-defined glucose and guanidine based copolymer, galactosylated 2-hydroxypropyl methacrylamide-s-3-guanidinopropyl methacrylamide (HPMA-s-GPMA) abbreviated as GGH was prepared and self-assembled with small hairpin RNA (shRNA) to inhibit human telomerase reverse transcriptase (hTERT) gene expression in vitro to develop a shRNA carrier. First, HPMA-s-APMA copolymers were synthesized by aqueous reversible addition-fragmentation chain transfer polymerization, followed by galactosylation and guanidinylation."
Our news editors obtained a quote from the research from Southeast University, "Then, three target shRNAs containing green fluorescent protein gene as a reporter were combined with GGH to form shRNA/GGH polyplexes. GGH copolymers could condense shRNA to form shRNA/GGH polyplex particles with a diameter in the range 122.8-331.6 nm in phosphate-buffered saline, and zeta potential values ranging from +3.7 to +16.5 mV at various charge ratios (N/P). That the cytotoxicity of GGH copolymers was significantly lower than that of PEI in human hepatocellular liver carcinoma cells (HepG2) and human cervix epithelial carcinoma cells. The transfection efficiency of shRNA/GGH polyplexes was higher than that of PEI at a charge ratio of 12 in the HepG2 cell line. Furthermore, shRNA/GGH polyplexes could effectively silence hTERT mRNA expression in serum-free medium (p
According to the news editors, the research concluded: "GGH copolymers could integrate advantages relating to galactose content for hepatocyte targeting, guanidino groups for cell penetration and HPMA component for shielding, showing great potential for effective hepatocyte targeting gene delivery."
For more information on this research see: Galactosylated 2-hydroxypropyl methacrylamide-s-3-guanidinopropyl methacrylamide copolymer as a small hairpin RNA carrier for inhibiting human telomerase reverse transcriptase expression. Journal of Gene Medicine, 2014;16(5-6):109-121. Journal of Gene Medicine can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Gene Medicine - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-2254)
The news editors report that additional information may be obtained by contacting Y. Wu, Southeast Univ, Key Lab Pharmaceut & Environm Engn, Sch Public Hlth, Nanjing 211189, Jiangsu, People's Republic of China. Additional authors for this research include J.K. Ji, R. Yang, X.Q. Zhang, Y.H. Li, Y.P. Pu and X.S. Li (see also Biotechnology).
Keywords for this news article include: Jiangsu, People's Republic of China, Asia, Biotechnology, Cancer Gene Therapy, Carrier Proteins, Enzymes and Coenzymes, Gastroenterology, Proteomics, Reverse Transcriptase, Ribonucleoproteins, Telomerase
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