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Investigators from University of Florida Zero in on Experimental Eye Research (Assessment of anti-scarring therapies in ex vivo organ cultured rabbit...

September 12, 2014



Investigators from University of Florida Zero in on Experimental Eye Research (Assessment of anti-scarring therapies in ex vivo organ cultured rabbit corneas)

By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Eye Research. According to news originating from Gainesville, Florida, by NewsRx correspondents, research stated, "The effects of a triple combination of siRNAs targeting key scarring genes were assessed using an ex vivo organ culture model of excimer ablated rabbit corneas. The central 6 mm diameter region of fresh rabbit globes was ablated to a depth of 155 microns with an excimer laser."

Our news journalists obtained a quote from the research from the University of Florida, "Corneas were excised, cultured at the air-liquid interface in defined culture medium supplemented with transforming growth factor beta 1 (TGFB1), and treated with either 1% prednisolone acetate or with 22.5 mu M cationic nanoparticles complexed with a triple combination of siRNAs (NP-siRNA) targeting TGFB1, TGFB Receptor (TGFBR2) and connective tissue growth factor (CTGF). Scar formation was measured using image analysis of digital images and levels of smooth muscle actin (SMA) were assessed in ablated region of corneas using qRT-PCR and immunostaining. Ex vivo cultured corneas developed intense haze-like scar in the wounded areas and levels of mRNAs for pro-fibrotic genes were significantly elevated 3-8 fold in wounded tissue compared to unablated corneas. Treatment with NP-siRNA or steroid significantly reduced quantitative haze levels by 55% and 68%, respectively, and reduced SMA mRNA and immunohistostaining. This ex vivo corneal culture system reproduced key molecular patterns of corneal scarring and haze formation generated in rabbits."

According to the news editors, the research concluded: "Treatment with NP-siRNAs targeting key scarring genes or an anti-inflammatory steroid reduced corneal haze and SMA mRNA and protein."

For more information on this research see: Assessment of anti-scarring therapies in ex vivo organ cultured rabbit corneas. Experimental Eye Research, 2014;125():173-182. Experimental Eye Research can be contacted at: Academic Press Ltd- Elsevier Science Ltd, 24-28 Oval Rd, London NW1 7DX, England. (Elsevier - www.elsevier.com; Experimental Eye Research - www.elsevier.com/wps/product/cws_home/622827)

The news correspondents report that additional information may be obtained from S. Sriram, University of Florida, Dept. of Mol Genet & Microbiol, Gainesville, FL 32611, United States. Additional authors for this research include D.J. Gibson, P. Robinson, L.Y. Pi, S. Tuli, A.S. Lewin and G. Schultz (see also Eye Research).

Keywords for this news article include: Gainesville, Florida, United States, North and Central America, Eye Research

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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