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Investigators from Faculty of Medicine Have Reported New Data on Enzymes and Coenzymes (A new self-assembled layer-by-layer glucose biosensor based...

September 8, 2014



Investigators from Faculty of Medicine Have Reported New Data on Enzymes and Coenzymes (A new self-assembled layer-by-layer glucose biosensor based on chitosan biopolymer entrapped enzyme with nitrogen doped graphene)

By a News Reporter-Staff News Editor at Clinical Trials Week -- New research on Enzymes and Coenzymes is the subject of a report. According to news reporting out of Brasov, Romania, by NewsRx editors, research stated, "The layer-by-layer (LbL) technique has been used for the construction of a new enzyme biosensor. Multilayer films containing glucose oxidase, GOx, and nitrogen-doped graphene (NG) dispersed in the biocompatible positively-charged polymer chitosan (chit(+)(NG + GOx)), together with the negatively charged polymer poly(styrene sulfonate), PSS-, were assembled by alternately immersing a gold electrode substrate in chit(+)(NG + GOx) and PSS- solutions."

Our news journalists obtained a quote from the research from the Faculty of Medicine, "Gravimetric monitoring during LbL assembly by an electrochemical quartz microbalance enabled investigation of the adsorption mechanism and deposited mass for each monolayer. Cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the LbL modified electrodes, in order to establish the contribution of each monolayer to the overall electrochemical properties of the biosensor. The importance of NG in the biosensor architecture was evaluated by undertaking a comparative study without NG in the chit layer. The GOx biosensor's analytical properties were evaluated by fixed potential chronoamperometry and compared with similar reported biosensors."

According to the news editors, the research concluded: "The biosensor operates at a low potential of -0.2 V vs., Ag/AgCl, exhibiting a high sensitivity of 10.5 mu A cm(-2) mM(-1), and a detection limit of 64 mu M. This study shows a simple approach in developing new biosensor architectures, combining the advantages of nitrogen-doped graphene with the LbL technique for enzyme immobilization."

For more information on this research see: A new self-assembled layer-by-layer glucose biosensor based on chitosan biopolymer entrapped enzyme with nitrogen doped graphene. Bioelectrochemistry, 2014;99():46-52. Bioelectrochemistry can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland. (Elsevier - www.elsevier.com; Bioelectrochemistry - www.elsevier.com/wps/product/cws_home/504080)

Our news journalists report that additional information may be obtained by contacting M.M. Barsan, Univ Transilvania Brasov, Fac Med, Brasov 500019, Romania. Additional authors for this research include M. David, M. Florescu, L. Tugulea and C.M.A. Brett (see also Enzymes and Coenzymes).

Keywords for this news article include: Brasov, Romania, Europe, Bioengineering, Bionanotechnology, Biosensing, Biotechnology, Chemicals, Chemistry, Electrochemical, Enzymes and Coenzymes, Nanobiotechnology, Nanotechnology, Nitrogen

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Clinical Trials Week


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