Findings on Biotechnology Discussed by Investigators at University of Natural Resources and Applied Life Science (Reducing Recon 2 for steady-state flux analysis of HEK cell culture)
By a News Reporter-Staff News Editor at Biotech Week -- New research on Technology is the subject of a report. According to news reporting originating from Vienna, Austria, by NewsRx correspondents, research stated, "A representative stoichiometric model is essential to perform metabolic flux analysis (MFA) using experimentally measured consumption (or production) rates as constraints. For Human Embryonic Kidney (HEK) cell culture, there is the opportunity to use an extremely well-curated and annotated human genome-scale model Recon 2 for MFA."
Our news editors obtained a quote from the research from the University of Natural Resources and Applied Life Science, "Performing MFA using Recon 2 without any modification would have implied that cells have access to all functionality encoded by the genome, which is not realistic. The majority of intracellular fluxes are poorly determined as only extracellular exchange rates are measured. This is compounded by the fact that there is no suitable metabolic objective function to suppress non-specific fluxes. We devised a heuristic to systematically reduce Recon 2 to emphasize flux through core metabolic reactions. This implies that cells would engage these dominant metabolic pathways to grow, and any significant changes in gross metabolic phenotypes would have invoked changes in these pathways."
According to the news editors, the research concluded: "The reduced metabolic model becomes a functionalized version of Recon 2 used for identifying significant metabolic changes in cells by flux analysis."
For more information on this research see: Reducing Recon 2 for steady-state flux analysis of HEK cell culture. Journal of Biotechnology, 2014;184():172-178. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Biotechnology - www.elsevier.com/wps/product/cws_home/505515)
The news editors report that additional information may be obtained by contacting L.E. Quek, Univ Nat Resources & Life Sci, Dept. of Biotechnol, Inst Appl Microbiol, Vienna, Austria. Additional authors for this research include S. Dietmair, M. Hanscho, V.S. Martinez, N. Borth and L.K. Nielsen (see also Technology).
Keywords for this news article include: Vienna, Austria, Europe, Technology
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