Findings in the Area of Small Interference RNAs (siRNAs) Reported from Case Western Reserve University (Multifunctional Cationic Lipid-Based Nanoparticles Facilitate Endosomal Escape and Reduction-Triggered Cytosolic siRNA Release)
By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Small Interference RNAs (siRNAs). According to news reporting out of Cleveland, Ohio, by NewsRx editors, research stated, "Small interfering RNA (siRNA) has garnered much attention in recent years as a promising avenue for cancer gene therapy due to its ability to silence diseaserelated genes. Effective gene silencing is contingent upon the delivery of siRNA into the cytosol of target cells and requires the implementation of delivery systems possessing multiple functionalities to overcome delivery barriers."
Our news journalists obtained a quote from the research from Case Western Reserve University, "The present work explores the multifunctional properties and biological activity of a recently developed cationic lipid carrier, (1-aminoethy0iminobis[N-(oleicylcysteiny1-1-amino-ethyppropionamide]) (ECO). The physicochemical properties and biological activity of ECO/siRNA nanoparticles were assessed over a range of N/P ratios to optimize the formulation. Potent and sustained luciferase silencing in a U87 glioblastoma cell line was observed, even in the presence of serum proteins. ECO/siRNA nanoparticles exhibited pH-dependent membrane disruption at pH levels corresponding to various stages of the intracellular trafficking pathway. It was found that disulfide linkages created during nanoparticle formation enhanced the protection of siRNA from degradation and facilitated site-specific siRNA release in the cytosol by glutathione-mediated reduction. Confocal microscopy confirmed that ECO/siRNA nanoparticles readily escaped from late endosomes prior to cytosolic release of the siRNA cargo."
According to the news editors, the research concluded: "These results demonstrate that the rationally designed multifunctionality of ECO/siRNA nanoparticles is critical for intracellular siRNA delivery and the continuing development of safe and effective delivery systems."
For more information on this research see: Multifunctional Cationic Lipid-Based Nanoparticles Facilitate Endosomal Escape and Reduction-Triggered Cytosolic siRNA Release. Molecular Pharmaceutics, 2014;11(8):2734-2744. Molecular Pharmaceutics can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Molecular Pharmaceutics - www.pubs.acs.org/journal/mpohbp)
Our news journalists report that additional information may be obtained by contacting M. Gujrati, Case Western Reserve University, Dept. of Biomed Engn, Cleveland, OH 44106, United States. Additional authors for this research include A. Malamas, T. Shin, E.L. Jin, Y.L. Sun and Z.R. Lu (see also Small Interference RNAs (siRNAs)).
Keywords for this news article include: Cleveland, Ohio, United States, North and Central America, Emerging Technologies, Genetics, Nanoparticle, Nanotechnology, Small Interference RNAs, Small Interference RNAs (siRNAs), siRNA
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