Findings from Shandong Agricultural University in Analytical Chemistry Reported (One-step, ultrasensitive, and electrochemical assay of microRNAs based on T7 exonuclease assisted cyclic enzymatic amplification)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Analytical Chemistry. According to news originating from Shandong, People's Republic of China, by NewsRx correspondents, research stated, "Taking advantage of the special exodeoxyribonuclease activity of T7 exonuclease, a simple, sensitive, selective, and label-free microRNA biosensor based on the cyclic enzymatic amplification method (CEAM) has been proposed. First, thiol functionalized DNA probes were assembled onto a gold nanoparticles modified gold electrode surface through a Au-S bond, followed by hybridizing with target miRNA."
Our news journalists obtained a quote from the research from Shandong Agricultural University, "Subsequently, DNA in RNA/DNA duplexes was digested by T7 exonuclease, which can release the microRNA molecules from the electrode surface and return into the buffer solution. Meanwhile, the released microRNA can further hybridize with the unhybridized DNA probes on the modified electrode surface. On the basis of it, an isothermal amplification cycle is realized. The T7 exonuclease-assisted CEAM achieved a low detection limit of 0.17 fM. Moreover, this assay presents excellent specificity with discriminating only a single-base mismatched microRNA sequence."
According to the news editors, the research concluded: "Furthermore, this work can also be applied to detect avian leukemia based on the decreased expression level of microRNA-21."
For more information on this research see: One-step, ultrasensitive, and electrochemical assay of microRNAs based on T7 exonuclease assisted cyclic enzymatic amplification. Analytical Chemistry, 2014;86(12):5606-10. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from M. Wang, College of Chemistry and Material Science, Shandong Agricultural University , Tai'an, Shandong 271018, People's Republic of China. Additional authors for this research include Z. Fu, B. Li, Y. Zhou, H. Yin and S. Ai (see also Analytical Chemistry).
Keywords for this news article include: Shandong, People's Republic of China, Asia, Analytical Chemistry.
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