Findings from Hong Kong University of Science and Technology Reveals New Findings on DNA Research (Noninvasive measurement of aristolochic acid-DNA adducts in urine samples from aristolochic acid-treated rats by liquid chromatography coupled ...)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in DNA Research. According to news reporting originating in Hong Kong, People's Republic of China, by NewsRx journalists, research stated, "Nephrotoxic aristolochic acids (AAs) form covalently bonded DNA adducts upon metabolic activation. In this work, a non-invasive approach to detect AAs exposure by quantifying urinary excreted DNA-AA adducts is presented."
The news reporters obtained a quote from the research from the Hong Kong University of Science and Technology, "The developed method entails solid-phase extraction (SPE) enrichment of the urine-excreted DNA-AAs adducts, addition of internal standard, and quantification by liquid chromatography coupled tandem mass spectrometric (LC-MS/MS) analysis. Quantitative analysis revealed 7-(deoxyadenosine-N-6-yl)-aristolactam II and 7-(deoxyguanosine-N-2-yl)-aristolactam I that were previously detected as major DNA-AA adducts in different organs of AA-dosed rats, were detected as the major urine excreted adducts. Lower levels of 7-(deoxyadenosine-N-6-yl)-aristolactam II and 7-(deoxyguanosine-N-2-yl)-aristolactam II were also detected in the collected urine samples. The identities of the detected urinary DNA-AA adducts were confirmed by comparing chromatographic retention time with synthetic standards, by high-accuracy MS, and MS/MS analyses. LC-MS/MS analysis of the urine samples collected from the AAs-dosed rats demonstrated a time-dependent decrease in the urinary adduct levels, indicating the urinary DNA-AA adduct levels were reflective of the tissue adduct levels."
According to the news reporters, the research concluded: "It is expected that the developed approach of detecting urinary DNA-AA adducts will facilitate further carcinogenesis investigations of AAs."
For more information on this research see: Noninvasive measurement of aristolochic acid-DNA adducts in urine samples from aristolochic acid-treated rats by liquid chromatography coupled tandem mass spectrometry: Evidence for DNA repair by nucleotide-excision repair mechanisms. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2014;766():1-6. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also DNA Research).
Our news correspondents report that additional information may be obtained by contacting E.M.K. Leung, Hong Kong University of Science & Technology, Dept. of Chem, Kowloon, Hong Kong, People's Republic of China.
Keywords for this news article include: Hong Kong, People's Republic of China, Asia, Aristolochic Acids, DNA Research, Hydrocarbons, Nitro Compounds, Phenanthrenes
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