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Data on Yersinia pestis Reported by J. Chenau and Co-Researchers (Detection of Yersinia pestis in environmental and food samples by intact cell...

September 9, 2014



Data on Yersinia pestis Reported by J. Chenau and Co-Researchers (Detection of Yersinia pestis in environmental and food samples by intact cell immunocapture and liquid chromatography-tandem mass spectrometry)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Proteobacteria have been published. According to news originating from Gif sur Yvette, France, by NewsRx correspondents, research stated, "Yersinia pestis is the causative agent of bubonic and pneumonic plague, an acute and often fatal disease in humans. In addition to the risk of natural exposure to plague, there is also the threat of a bioterrorist act, leading to the deliberate spread of the bacteria in the environment or food."

Our news journalists obtained a quote from the research, "We report here an immuno-liquid chromatography-tandem mass spectrometry (immuno-LC-MS/MS) method for the direct (i.e., without prior culture), sensitive, and specific detection of Y. pestis in such complex samples. In the first step, a bottom-up proteomics approach highlighted three relevant protein markers encoded by the Y. pestis-specific plasmids pFra (murine toxin) and pPla (plasminogen activator and pesticin). Suitable proteotypic peptides were thoroughly selected to monitor the three protein markers by targeted MS using the selected reaction monitoring (SRM) mode. Immunocapture conditions were optimized for the isolation and concentration of intact bacterial cells from complex samples. The immuno-LC-SRM assay has a limit of detection of 2 x 10(4) CFU/mL in milk or tap water, which compares well with those of state-of-the-art immunoassays."

According to the news editors, the research concluded: "Moreover, we report the first direct detection of Y. pestis in soil, which could be extremely useful in confirming Y. pestis persistence in the ground."

For more information on this research see: Detection of Yersinia pestis in environmental and food samples by intact cell immunocapture and liquid chromatography-tandem mass spectrometry. Analytical Chemistry, 2014;86(12):6144-52. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

The news correspondents report that additional information may be obtained from J. Chenau, Service de Pharmacologie et d'Immunoanalyse, Institut de Biologie et de Technologies de Saclay (iBiTec-S), Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA) , 91191 Gif-sur-Yvette, France. Additional authors for this research include F. Fenaille, S. Simon, S. Filali, H. Volland, C. Junot, E. Carniel and F. Becher (see also Proteobacteria).

Keywords for this news article include: Gif sur Yvette, France, Europe, Enterobacteriaceae, Gram Negative Bacteria, Proteobacteria, Yersinia pestis.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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