Data on Salmonella Discussed by Researchers at Hunan Normal University (High Specific DNAzyme-Aptamer Sensor for Salmonella paratyphi A Using Single-Walled Nanotubes-Based Dual Fluorescence-Spectrophotometric Methods)
By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Proteobacteria. According to news reporting out of Changsha, People's Republic of China, by NewsRx editors, research stated, "In this work, single-stranded DNA aptamers that are highly specific to enterotoxigenic Salmonella paratyphi A were obtained from an enriched oligonucleotide pool using Systematic Evolution of Ligands by Exponential Enrichment (SELEX) to target the flagellin protein. The selected aptamers were confirmed to have high sensitivity and specificity to the flagellin."
Our news journalists obtained a quote from the research from Hunan Normal University, "In addition, a probe (P0) containing the DNAzyme and fluorescein isothiocyanate-labeled aptamer3 sequences was employed as a dual probe for observing fluorescence and absorbance changes. The flagellin demonstrated low detection limits of 5 ng/mL by fluorescence and 20 ng/mL by spectrophotometry. Moreover, milk samples spiked with Salmonella paratyphi A were also detected, with the low detection limits increasing to 10(5) CFU/mL by fluorescence and 10(6) CFU/mL by spectrophotometry."
According to the news editors, the research concluded: "The combination of fluorescence and spectrophotometry offers a specific, rapid, and sensitive way for detecting Salmonella paratyphi A and has potential for detecting other pathogens in food."
For more information on this research see: High Specific DNAzyme-Aptamer Sensor for Salmonella paratyphi A Using Single-Walled Nanotubes-Based Dual Fluorescence-Spectrophotometric Methods. Journal of Biomolecular Screening, 2014;19(7):1099-1106. Journal of Biomolecular Screening can be contacted at: Sage Publications Inc, 2455 Teller Rd, Thousand Oaks, CA 91320, USA. (Sage Publications - www.sagepub.com/; Journal of Biomolecular Screening - jbx.sagepub.com)
Our news journalists report that additional information may be obtained by contacting Y. Ning, Hunan Normal Univ, Changsha 410081, Hunan, People's Republic of China. Additional authors for this research include W.K. Li, Y.F. Duan, M. Yang and L. Deng (see also Proteobacteria).
Keywords for this news article include: Changsha, People's Republic of China, Asia, Emerging Technologies, Enterobacteriaceae, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Nanotechnology, Nanotube, Salmonella, Salmonellosis
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