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Data on Enzymes and Coenzymes Detailed by Researchers at Southeast University (Enhanced Enzymatic Reactivity for Electrochemically Driven Drug...

September 9, 2014



Data on Enzymes and Coenzymes Detailed by Researchers at Southeast University (Enhanced Enzymatic Reactivity for Electrochemically Driven Drug Metabolism by Confining Cytochrome P450 Enzyme in TiO2 Nanotube Arrays)

By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Enzymes and Coenzymes have been presented. According to news originating from Jiangsu, People's Republic of China, by NewsRx correspondents, research stated, "Understanding the enzymatic reaction kinetics that occur within a confined space or interface is a significant challenge. Herein, a nanotube array enzymatic reactor (CYP2C9/Au/TNA) was constructed by electrostatically adsorbing enzyme on the inner wall of TiO2 nanotube arrays (TNAs)."

Our news journalists obtained a quote from the research from Southeast University, "TNAs with different dimensions could be fabricated by the anodization of titanium foil through varying the anodization potential or time. The electrical conductivity of TNAs was improved by electrodepositing Au nanoparticles on the inner wall of TNAs. The cytochrome P450 2C9 enzyme (CYP2C9) was confined inside TNAs as a model. The enzymatic activity of CYP2C9 and tolbutamide metabolic yield could be effectively regulated by changing the nanotube diameter and length of TNAs. The enzymatic rate constant k(cat) and apparent Michaelis constant K-m(app) were determined to be 9.89 s(-1) and 4.8 mu M at the tube inner diameter of about 64 nm and length of 1.08 mu m. The highest metabolic yield of tolbutamide reached 14.6%."

According to the news editors, the research concluded: "Furthermore, the designed nanotube array enzymatic reactor could be also used in situ to monitor the tolbutamide concentration with sensitivity of 28.8 mu A mM(-1) and detection limit of 0.52 mu M. Therefore, the proposed nanotube array enzymatic reactor was a good vessel for studying enzyme biocatalysis and drug metabolism, and has potential applications including efficient biosensors and bioreactors for chemical synthesis."

For more information on this research see: Enhanced Enzymatic Reactivity for Electrochemically Driven Drug Metabolism by Confining Cytochrome P450 Enzyme in TiO2 Nanotube Arrays. Analytical Chemistry, 2014;86(15):8003-8009. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

The news correspondents report that additional information may be obtained from J.S. Lu, Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, People's Republic of China. Additional authors for this research include H.N. Li, D.M. Cui, Y.J. Zhang and S.Q. Liu (see also Enzymes and Coenzymes).

Keywords for this news article include: Jiangsu, People's Republic of China, Asia, Chemicals, Chemistry, Cytochromes, Electrochemical, Enzymes and Coenzymes, Hemeproteins

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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