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Data on Biomolecular Screening Reported by Researchers at Novartis Pharma (Fluorescence Lifetime-Based Competitive Binding Assays for Measuring the...

September 10, 2014



Data on Biomolecular Screening Reported by Researchers at Novartis Pharma (Fluorescence Lifetime-Based Competitive Binding Assays for Measuring the Binding Potency of Protease Inhibitors In Vitro)

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Biotechnology have been published. According to news reporting originating in Basel, Switzerland, by NewsRx journalists, research stated, "Fluorescence lifetime (FLT)-based assays have developed to become highly attractive tools in drug discovery. All recently published examples of FLT-based assays essentially describe their use for monitoring enzyme-mediated peptide modifications, such as proteolytic cleavage or phosphorylation/dephosphorylation."

The news reporters obtained a quote from the research from Novartis Pharma, "Here we report the development of competitive binding assays as novel, inhibitor-centric assays, principally employing the FLT of the acridone dye Puretime 14 (PT14) as the readout parameter. Exemplified with two case studies on human serine proteases, the details of the rationale for both the design and synthesis of probes (i.e., active site-directed low-molecular-weight inhibitors conjugated to PT14) are provided."

According to the news reporters, the research concluded: "Data obtained from testing inhibitors with the novel assay format match those obtained with alternative formats such as FLT-based protease activity and time-resolved fluorescence resonance energy transfer-based competitive binding assays."

For more information on this research see: Fluorescence Lifetime-Based Competitive Binding Assays for Measuring the Binding Potency of Protease Inhibitors In Vitro. Journal of Biomolecular Screening, 2014;19(6):870-877. Journal of Biomolecular Screening can be contacted at: Sage Publications Inc, 2455 Teller Rd, Thousand Oaks, CA 91320, USA. (Sage Publications - www.sagepub.com/; Journal of Biomolecular Screening - jbx.sagepub.com)

Our news correspondents report that additional information may be obtained by contacting A. Boettcher, Novartis Pharma AG, Global Discovery Chem, NIBR, CH-4002 Basel, Switzerland. Additional authors for this research include N. Gradoux, E. Lorthiois, T. Brandl, D. Orain, N. Schiering, F. Cumin, J. Woelcke and U. Hassiepen (see also Biotechnology).

Keywords for this news article include: Basel, Switzerland, Europe, Biotechnology, Enzymes and Coenzymes, Protease

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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