News Column

Report Summarizes Polyethylene Glycols Study Findings from University of Siegen (Enzyme-Sensing Chitosan Hydrogels)

August 15, 2014

By a News Reporter-Staff News Editor at Science Letter -- A new study on Polyethylene Glycols is now available. According to news originating from Siegen, Germany, by NewsRx correspondents, research stated, "We report on a chitosan hydrogel-based platform for the detection of enzymes, which is compatible with the implementation in infection-sensing wound dressings. Thin films of the established wound dressing biopolymer chitosan were functionalized with a fluorogenic substrate, which is released upon enzymatic degradation, resulting in a pronounced increase in fluorescence emission intensity."

Our news journalists obtained a quote from the research from the University of Siegen, "In this first model study, the fluorogenic substrate alanyl-alanyl-phenylalanine-7-amido-4-methylcoumarin (AAP-AMC) was covalently conjugated via amide bond formation to chitosan and was shown to facilitate the detection of the serine protease a-chymotrypsin. Systematic investigations established the dependence of hydrogel thickness and substrate loading on the hydrogel preparation conditions, as well as the dependence of the rate of the reaction on the initial enzyme concentration and the loading of AAP-AMC in the hydrogel. The initial release rate of the fluorophore 7-AMC was found to be linear with enzyme concentration and substrate loading and was independent of hydrogel thickness. Under optimized conditions the hydrogel reports the presence of a-chymotrypsin in

According to the news editors, the research concluded: "This generic approach, which can be adapted to detect different kinds of enzymes by using appropriate fluorogenic or chromogenic substrates, is highly interesting for targeting the detection of specific pathogenic bacteria, e.g., in wound dressings."

For more information on this research see: Enzyme-Sensing Chitosan Hydrogels. Langmuir, 2014;30(26):7842-7850. Langmuir can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Langmuir -

The news correspondents report that additional information may be obtained from M.M.S. Ebrahimi, University of Siegen, Dept. of Biol & Chem, D-57076 Siegen, Germany (see also Polyethylene Glycols).

Keywords for this news article include: Siegen, Europe, Germany, Alcohols, Hydrogel, Organic Chemicals, Polyethylene Glycols, Enzymes and Coenzymes

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Source: Science Letter

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