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New Separation Science Study Results Reported from M. Chen et al (Preparative isolation and analysis of alcohol dehydrogenase inhibitors from...

August 15, 2014



New Separation Science Study Results Reported from M. Chen et al (Preparative isolation and analysis of alcohol dehydrogenase inhibitors from Glycyrrhiza uralensis root using ultrafiltration combined with high-performance liquid chromatography ...)

By a News Reporter-Staff News Editor at Science Letter -- Research findings on Science are discussed in a new report. According to news reporting from Changsha, People's Republic of China, by NewsRx journalists, research stated, "A simple, rapid, and effective assay based on ultrafiltration combined with high-performance liquid chromatography and high-speed countercurrent chromatography was developed for screening and purifying alcohol dehydrogenase inhibitors from Glycyrrhiza uralensis root extract. Experiments were carried out to optimize binding conditions including alcohol dehydrogenase concentration, incubation time, temperature, and pH."

The news correspondents obtained a quote from the research, "By comparing the chromatograms, three compounds were found possessing alcohol dehydrogenase binding activity in Glycyrrhiza uralensis root. Under the target-guidance of ultrafiltration combined with the high-performance liquid chromatography experiment, liquiritin (1), isoliquiritin (2), and liquiritigenin (3) were separated by high-speed countercurrent chromatography using ethyl acetate/methanol/water (5:1:4) as the solvent system."

According to the news reporters, the research concluded: "The alcohol dehydrogenase inhibitory activities of these three isolated compounds were assessed; compound 2 showed strongest inhibitory activity with an IC50 of 8.95 mu M. The results of the present study indicated that the combinative method using ultrafiltration, high-performance liquid chromatography and high-speed countercurrent chromatography could be widely applied for the rapid screening and isolation of enzyme inhibitors from complex mixtures."

For more information on this research see: Preparative isolation and analysis of alcohol dehydrogenase inhibitors from Glycyrrhiza uralensis root using ultrafiltration combined with high-performance liquid chromatography and high-speed countercurrent chromatography. Journal of Separation Science, 2014;37(13):1546-1551. Journal of Separation Science can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Journal of Separation Science - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1615-9314)

Our news journalists report that additional information may be obtained by contacting M. Chen, Collaborat Innovat Center Resource Conserving & Envi, Changsha, Hunan, People's Republic of China. Additional authors for this research include L.L. Liu and X.Q. Chen (see also Science).

Keywords for this news article include: Asia, Science, Changsha, Imaging Technology, People's Republic of China, High-Performance Liquid Chromatography

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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