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Study Findings from Harbin Institute of Technology Broaden Understanding of DNA Research (A hot start alternative for high-fidelity DNA polymerase...

August 12, 2014



Study Findings from Harbin Institute of Technology Broaden Understanding of DNA Research (A hot start alternative for high-fidelity DNA polymerase amplification mediated by quantum dots)

By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on DNA Research are discussed in a new report. According to news reporting out of Weihai, People's Republic of China, by NewsRx editors, research stated, "Quantum dots (QDs) are of great interest due to their unique chemical and physical properties. Recently, a hot start (HS) polymerase chain reaction (PCR) amplification performance based on QDs with a high-fidelity Pfu DNA polymerase has been reported."

Our news journalists obtained a quote from the research from the Harbin Institute of Technology, "However, whether QDs can trigger HS effects with other high-fidelity or conventional DNA polymerases is yet to be understood. In the present study, we studied the QD-triggered HS effects with four high-fidelity and three conventional DNA polymerases, and the HS effect comparisons among them were also made. It was found that QDs could trigger a distinct HS PCR amplification performance with all the four tested high-fidelity DNA polymerases, and specific target DNA could be well amplified even if the PCR mixture was pre-incubated for 2 h at 50C. On the contrary, the HS effects were not prominent with all the three conventional Taq DNA polymerases. Specifically, the fidelity of Pfu is not sacrificed in the presence of QDs, even after a 1 h pre-incubation at 50C before PCR."

According to the news editors, the research concluded: "Furthermore, the electrophoresis results preliminarily demonstrated that QDs prefer to adsorb high-fidelity polymerases rather than conventional ones, which might result in the QD-triggered HS effects on PCR performance by using high-fidelity DNA polymerases."

For more information on this research see: A hot start alternative for high-fidelity DNA polymerase amplification mediated by quantum dots. Acta Biochimica Et Biophysica Sinica, 2014;46(6):502-11. (Wiley-Blackwell - www.wiley.com/; Acta Biochimica Et Biophysica Sinica - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1745-7270)

Our news journalists report that additional information may be obtained by contacting F. Sang, School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, People's Republic of China. Additional authors for this research include Y. Yang, Y. Lin and Z. Zhang (see also DNA Research).

Keywords for this news article include: Asia, Weihai, DNA Research, Quantum Dots, Quantum Physics, People's Republic of China.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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