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Studies from University of Cambridge Have Provided New Data on Green Fluorescent Protein (A Tailor-Made "Tag-Receptor" Affinity Pair for the...

August 12, 2014



Studies from University of Cambridge Have Provided New Data on Green Fluorescent Protein (A Tailor-Made "Tag-Receptor" Affinity Pair for the Purification of Fusion Proteins)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Proteins. According to news reporting from Cambridge, United Kingdom, by NewsRx journalists, research stated, "A novel affinity 'tag-receptor' pair was developed as a generic platform for the purification of fusion proteins. The hexapeptide RKRKRK was selected as the affinity tag and fused to green fluorescent protein (GFP)."

The news correspondents obtained a quote from the research from the University of Cambridge, "The DNA fragments were designed, cloned in Pet-21c expression vector and expressed in E. coli host as soluble protein. A solid-phase combinatorial library based on the Ugi reaction was synthesized: 64 affinity ligands displaying complementary functionalities towards the designed tag. The library was screened by affinity chromatography in a 96-well format for binding to the RKRKRK-tagged GFP protein. Lead ligand A7C1 was selected for the purification of RKRKRK fusion proteins. The affinity pair RKRKRK-tagged GFP with A7C1 emerged as a promising solution (K-a of 2.45 x 10(5)m(-1))."

According to the news reporters, the research concluded: "The specificity of the ligand towards the tag was observed experimentally and theoretically through automated docking and molecular dynamics simulations."

For more information on this research see: A Tailor-Made "Tag-Receptor" Affinity Pair for the Purification of Fusion Proteins. Chembiochem, 2014;15(10):1423-1435. Chembiochem can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Chembiochem - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1439-7633)

Our news journalists report that additional information may be obtained by contacting A.S. Pina, University of Cambridge, Dept. of Chem Engn & Biotechnol, Inst Biotechnol, Cambridge CB2 1QT, United Kingdom. Additional authors for this research include M. Guilherme, A.S. Pereira, C. Fernandes, R.J.F. Branco, G. El Khoury, C.R. Lowe and A.C.A. Roque (see also Proteins).

Keywords for this news article include: Europe, Cambridge, United Kingdom, Green Fluorescent Protein

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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