Researchers from National Center for Scientific Research (CNRS) Report Recent Findings in General Chemistry (Speciation of iodine-containing proteins in Nori seaweed by gel electrophoresis laser ablation ICP-MS)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on General Chemistry have been published. According to news originating from Pau, France, by NewsRx correspondents, research stated, "An analytical approach providing an insight into speciation of iodine in water insoluble fraction of edible seaweed (Nori) was developed. The seaweed, harvested in the Galician coast (Northwestern Spain), contained 67.7 +/- 1.3 mu g g(-1) iodine of which 25% was water soluble and could be identifies as iodide."
Our news journalists obtained a quote from the research from National Center for Scientific Research (CNRS), "Extraction conditions of water insoluble residue using urea, NaOH, SDS and Triton X-100 were investigated. The protein pellets obtained in optimized conditions (after precipitation of urea extracts with acetone), were digested with trypsin and protease XIV. Size exclusion chromatography-ICP-MS of both enzymatic digests demonstrated the occurrence of iodoaminoacids putatively present in proteins. Intact proteins could be separated by gel electrophoresis after an additional extraction of the protein extract with phenol."
According to the news editors, the research concluded: "Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) with laser ablation ICP-MS detection of I-127 indicated the presence of iodine in protein bands corresponding to molecular masses of 110 kDa, 40 kDa, 27 kDa, 20 kDa and 10 kDa. 2D IEF-SDS PAGE with laser ablation ICP-MS I-127 imaging allowed the detection of 5 iodine containing protein spots in the alkaline pI range."
For more information on this research see: Speciation of iodine-containing proteins in Nori seaweed by gel electrophoresis laser ablation ICP-MS. Talanta, 2014;127():175-180. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
The news correspondents report that additional information may be obtained from V. Romaris-Hortas, CNRS UPPA, Lab Chim Analyt Bioinorgan & Environm, UMR5254, F-64053 Pau, France. Additional authors for this research include J. Bianga, A. Moreda-Pineiro, P. Bermejo-Barrera and J. Szpunar (see also General Chemistry).
Keywords for this news article include: Pau, France, Europe, General Chemistry
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