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Researchers from Hamdard University Describe Findings in Sulfur Amino Acids (Genetically-encoded nanosensor for quantitative monitoring of methionine...

August 12, 2014



Researchers from Hamdard University Describe Findings in Sulfur Amino Acids (Genetically-encoded nanosensor for quantitative monitoring of methionine in bacterial and yeast cells)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Sulfur Amino Acids have been published. According to news reporting originating in New Delhi, India, by NewsRx journalists, research stated, "Metabolic engineering of microorganisms for production of biological molecules represent a key goal for industrial biotechnology. The metabolic engineering requires detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo."

The news reporters obtained a quote from the research from Hamdard University, "Genetically-encoded fluorescence resonance energy transfer (FRET) sensors represent a promising technology for measuring metabolite levels and corresponding rate changes in live cells. In the present paper, we report the development of genetically-encoded FRET-based nanosensor for methionine as metabolic engineering of microbial strains for the production of l-methionine is of major interest in industrial biotechnology. In this nanosensor, methionine binding protein (MetN) from Escherichia coli (E. coli) K12 was taken and used as the reporter element of the sensor. The MetN was sandwiched between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP). Specificity, affinity, pH stability and metal effects was analyzed for the in vitro characterization of this nanosensor, named as FLIPM. The FLIPM is very specific to methionine and found to be stable with the pH within the physiological range. The calculated affinity (Kd) of FLIPM was 203 M. This nanosensor successfully monitored the intracellular level of methionine in bacterial as well as yeast cell."

According to the news reporters, the research concluded: "The data suggest that these nanosensors may be a versatile tool for studying the in vivo dynamics of methionine level non-invasively in living cells."

For more information on this research see: Genetically-encoded nanosensor for quantitative monitoring of methionine in bacterial and yeast cells. Biosensors & Bioelectronics, 2014;59():358-64. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)

Our news correspondents report that additional information may be obtained by contacting M. Mohsin, Molecular Ecology Laboratory, Dept. of Botany, Jamia Hamdard (Hamdard University), New Delhi 110062, India (see also Sulfur Amino Acids).

Keywords for this news article include: Asia, India, New Delhi, Methionine, Sulfur Amino Acids, Neutral Amino Acids, Essential Amino Acids, Metabolic Engineering.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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