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Researchers at Washington University Release New Data on Marine Genomics [Sequencing and characterization of the anadromous steelhead (Oncorhynchus...

August 11, 2014



Researchers at Washington University Release New Data on Marine Genomics [Sequencing and characterization of the anadromous steelhead (Oncorhynchus mykiss) transcriptome]

By a News Reporter-Staff News Editor at Biotech Business Week -- Fresh data on Life Science Research are presented in a new report. According to news reporting out of St. Louis, Missouri, by NewsRx editors, research stated, "Identifying the traits that differ between hatchery and wild fish may allow for pragmatic changes to hatchery practice. To meet those ends, we sequenced, assembled, and characterized the anadromous steelhead (Oncorhynchus mykiss) transcriptome."

Our news journalists obtained a quote from the research from Washington University, "Using the Illumina sequencing platform, we sequenced nearly 41 million 76-mer reads representing 3.1 Gbp of steelhead transcriptome. Upon final assembly, this sequence data yielded 86,402 transcript scaffolds, of which, 66,530 (77%) displayed homology to proteins of the non-redundant NCBI database. Gene descriptions and gene ontology terms were used to annotate the transcriptome resulting in 4030 unique gene ontology (GO) annotations attributed to the assembled sequences. We also conducted a comparative analysis that identified homologous genes within four other fish species including zebrafish (Danio rerio), stickleback (Gasterosteus aculeatus), and two pufferfish species (Tetraodon nigroviridis and Takifugu rubripes). Comparing our steelhead reference assembly directly to the transcriptome for rainbow trout (the fresh water life-history variant of the same species) revealed that while the steelhead and rainbow trout transcriptomes are complementary, the steelhead data will be useful for investigating questions related to anadromous (ocean-going) fishes."

According to the news editors, the research concluded: "These sequence data and web tools provide a useful set of resources for salmonid researchers and the broader genomics community (available at http://salmon.cgrb.oregonstate.edu)."

For more information on this research see: Sequencing and characterization of the anadromous steelhead (Oncorhynchus mykiss) transcriptome. Marine Genomics, 2014;15():13-15. Marine Genomics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Marine Genomics - www.elsevier.com/wps/product/cws_home/713752)

Our news journalists report that additional information may be obtained by contacting S.E. Fox, Washington University, Div Biol & Biomed Sci, St Louis, MO 63110, United States. Additional authors for this research include M.R. Christie, M. Marine, H.D. Priest, T.C. Mockler and M.S. Blouin (see also Life Science Research).

Keywords for this news article include: Missouri, St. Louis, United States, Life Science Research, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Business Week


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