Researchers at Spanish National Research Council (CSIC) Report New Data on Proteomics (Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: A redox proteomic investigation)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Proteomics are presented in a new report. According to news reporting from Cadiz, Spain, by NewsRx journalists, research stated, "Relatively little is known about the fate and effects of nanomaterials even in relatively simple organisms such as Mytilus edulis. Here, copper oxide nanoparticles (CuO NP) are shown to induce dose-dependent toxic effects at the biochemical, physiological and tissue levels in the blue mussel."
The news correspondents obtained a quote from the research from Spanish National Research Council (CSIC), "Stable CuO NP suspensions were sized by differential light scattering and nanoparticle tracking analysis to yield average particle diameters of approximately 100 nm. These were administered to M. edulis, at doses of 400, 700 and 1000 ppb. Ingested copper was predominantly located in the gill tissue with small amounts in digestive gland. Fifteen coomassie-stained spots were excised from two dimensional gel electrophoresis separations of gill tissue extacts and identified by peptide mass fingerprinting. These contained six unique proteins (alpha- and beta-tubulin, actin, tropomyosin, triosephosphate isomerase and Cu-Zn superoxide dismutase). Of these, two spots (actin and triosephosphate isomerase) showed decreased protein thiols while three (alpha-tubulin, tropomyosin and Cu-Zn superoxide dismutase) showed increased carbonylation which is indicative of protein oxidation of cytoskeleton and enzymes in response to CuO NP. The neutral red retention time (NRRT) assay revealed toxicity due to the CuO NPs which was comparable with toxic metal oxide nanoparticles such as chromium and cobalt. In contrast, non-toxic titanium and gold metal oxide nanoparticles gave no NRRT effects at similar NP concentrations."
According to the news reporters, the research concluded: "Histology revealed deposition of pigmented brown cells in response to CuO NP, located predominantly along the mantle and gill margin but also lining digestive tubules and some of the sinuses and distributed throughout the connective tissue and in the adductor muscle."
For more information on this research see: Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: A redox proteomic investigation. Chemosphere, 2014;108():289-299. Chemosphere can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Chemosphere - www.elsevier.com/wps/product/cws_home/362)
Our news journalists report that additional information may be obtained by contacting W.T. Hu, Inst Ciencias Marinas Andalucia CSIC, Cadiz, Spain. Additional authors for this research include S. Culloty, G. Darmody, S. Lynch, J. Davenport, S. Ramirez-Garcia, K.A. Dawson, I. Lynch, J. Blasco and D. Sheehan (see also Proteomics).
Keywords for this news article include: Cadiz, Spain, Europe, Dismutase, Proteomics, Tropomyosin, Nanoparticle, Nanotechnology, Emerging Technologies, Enzymes and Coenzymes, Microfilament Proteins
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