Researchers at Cornell University Release New Data on Tissue Engineering (3-D Intestinal Scaffolds for Evaluating the Therapeutic Potential of Probiotics)
By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Biomedicine and Biomedical Engineering have been presented. According to news reporting originating from Ithaca, New York, by NewsRx correspondents, research stated, "Biomimetic in vitro intestinal models are becoming useful tools for studying host-microbial interactions. In the past, these models have typically been limited to simple cultures on 2-D scaffolds or Transwell inserts, but it is widely understood that epithelial cells cultured in 3-D environments exhibit different phenotypes that are more reflective of native tissue, and that different microbial species will preferentially adhere to select locations along the intestinal villi."
Our news editors obtained a quote from the research from Cornell University, "We used a synthetic 3-D tissue scaffold with villous features that could support the coculture of epithelial cell types with select bacterial populations. Our end goal was to establish microbial niches along the crypt-villus axis in order to mimic the natural microenvironment of the small intestine, which could potentially provide new insights into microbe-induced intestinal disorders, as well as enabling targeted probiotic therapies. We recreated the surface topography of the small intestine by fabricating a biodegradable and biocompatible vinous scaffold using poly lactic-glycolic acid to enable the culture of Caco-2 with differentiation along the crypt-villus axis in a similar manner to native intestines. This was then used as a platform to mimic the adhesion and invasion profiles of both Salmonella and Pseudomonas, and assess the therapeutic potential of Lactobacillus and commensal Escherichia coli in a 3-D setting."
According to the news editors, the research concluded: "We found that, in a 3-D environment, Lactobacillus is more successful at displacing pathogens, whereas Nissle is more effective at inhibiting pathogen adhesion."
For more information on this research see: 3-D Intestinal Scaffolds for Evaluating the Therapeutic Potential of Probiotics. Molecular Pharmaceutics, 2014;11(7):2030-2039. Molecular Pharmaceutics can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Molecular Pharmaceutics - www.pubs.acs.org/journal/mpohbp)
The news editors report that additional information may be obtained by contacting C.M. Costello, Cornell University, Dept. of Biol & Environm Engn, Ithaca, NY 14853, United States. Additional authors for this research include R.M. Sorna, Y.L. Goh, I. Cengic, N.K. Jain and J.C. March (see also Biomedicine and Biomedical Engineering).
Keywords for this news article include: Tissue Engineering, Biomedicine and Biomedical Engineering, Ithaca, New York, United States, Bioengineering, North and Central America
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