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Reports on Cancer Therapy from Annamalai University Provide New Insights [Enhanced cytotoxicity and apoptosis-induced anticancer effect of...

August 13, 2014



Reports on Cancer Therapy from Annamalai University Provide New Insights [Enhanced cytotoxicity and apoptosis-induced anticancer effect of silibinin-loaded nanoparticles in oral carcinoma (KB) cells]

By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Oncology. According to news reporting out of Tamil Nadu, India, by NewsRx editors, research stated, "Silibinin (SIL) is a plant derived flavonoid isolated from the fruits and seeds of the milk thistle (Silybum marianum). Silibinin possesses a wide variety of biological applications including anticancer activities but poor aqueous solubility and poor bioavailability limit its potential and efficacy at the tumor sites."

Our news journalists obtained a quote from the research from Annamalai University, "In the present study, silibinin was encapsulated in Eudragit E (EE) nanoparticles in the presence of stabilizing agent polyvinyl alcohol (PVA) and its anticancer efficacy in oral carcinoma (KB) cells was studied. Silibinin loaded nanoparticles (SILNPs) were prepared by nanoprecipitation technique and characterized in terms of size distribution, morphology, surface charge, encapsulation efficiency and in vitro drug release. MTT assay revealed higher cytotoxic efficacy of SILNPs than free SIL in KB cells. Meanwhile, reactive oxygen species (ROS) determination revealed the significantly higher intracellular ROS levels in SILNPs treated cells compared to free SIL treated cells. Therefore, the differential cytotoxicity between SILNPs and SIL may be mediated by the discrepancy of intracellular ROS levels. Moreover, acridine orange (AO) and ethidium bromide (EB) dual staining and reduced mitochondrial membrane potential (MMP) confirmed the induction of apoptosis with nanoparticle treatment. Further, the extent of DNA damage (evaluated by comet assay) was significantly increased in SILNPs than free SIL in KB cells."

According to the news editors, the research concluded: "Taken together, the present study suggests that silibinin-loaded nanoparticles can be used as an effective drug delivery system to produce a better chemopreventive response for the treatment of cancer."

For more information on this research see: Enhanced cytotoxicity and apoptosis-induced anticancer effect of silibinin-loaded nanoparticles in oral carcinoma (KB) cells. Materials Science & Engineering C-Materials for Biological Applications, 2014;41():274-282. Materials Science & Engineering C-Materials for Biological Applications can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Oncology).

Our news journalists report that additional information may be obtained by contacting M. Gohulkumar, Annamalai Univ, Dept. of Biochem & Biotechnol, Annamalainagar 608002, Tamil Nadu, India. Additional authors for this research include K. Gurushankar, N.R. Prasad and N. Krishnakumar.

Keywords for this news article include: Asia, India, Therapy, KB Cells, Oncology, Carcinoma, Cell Line, Tamil Nadu, Hela Cells, Nanoparticle, Nanotechnology, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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