Report Summarizes Biomacromolecules Study Findings from Loughborough University (Anomalous small angle x-ray scattering simulations: proof of concept for distance measurements for nanoparticle-labelled biomacromolecules in solution)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Biomacromolecules is now available. According to news reporting from Loughborough, United Kingdom, by NewsRx journalists, research stated, "Anomalous small angle X-ray scattering can in principle be used to determine distances between metal label species on biological molecules. Previous experimental studies in the past were unable to distinguish the label-label scattering contribution from that of the molecule, because of the use of atomic labels; these labels contribute only a small proportion of the total scattering signal."
The news correspondents obtained a quote from the research from Loughborough University, "However, with the development of nanocrystal labels (of 50-100 atoms) there is the possibility for a renewed attempt at applying anomalous small angle X-ray scattering for distance measurement. This is because the contribution to the scattered signal is necessarily considerably stronger than for atomic labels. Here we demonstrate through simulations, the feasibility of the technique to determine the end-to-end distances of labelled nucleic acid molecules as well as other internal distances mimicking a labelled DNA binding protein if the labels are dissimilar metal nanocrystals. Of crucial importance is the ratio of mass of the nanocrystals to that of the labelled macromolecule, as well as the level of statistical errors in the scattering intensity measurements."
According to the news reporters, the research concluded: "The mathematics behind the distance determination process is presented, along with a fitting routine than incorporates maximum entropy regularisation."
For more information on this research see: Anomalous small angle x-ray scattering simulations: proof of concept for distance measurements for nanoparticle-labelled biomacromolecules in solution. Plos One, 2014;9(4):e95664. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting V.J. Pinfield, Chemical Engineering Department, Loughborough University, Loughborough, Leicestershire, UK (see also Biomacromolecules).
Keywords for this news article include: Europe, Loughborough, United Kingdom, Biomacromolecules.
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