Recent Reports from Georgia Institute of Technology and Emory University Highlight Findings in Molecular Biotechnology (Systematic optimization of multiplex zymography protocol to detect active cathepsins K, L, S, and V in healthy and diseased ...)
By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Biotechnology have been presented. According to news originating from Atlanta, Georgia, by NewsRx correspondents, research stated, "Cysteine cathepsins are a family of proteases identified in cancer, atherosclerosis, osteoporosis, arthritis, and a number of other diseases. As this number continues to rise, so does the need for low cost, broad use quantitative assays to detect their activity and can be translated to the clinic in the hospital or in low resource settings."
Our news journalists obtained a quote from the research from the Georgia Institute of Technology and Emory University, "Multiplex cathepsin zymography is one such assay that detects subnanomolar levels of active cathepsins K, L, S, and V in cell or tissue preparations observed as clear bands of proteolytic activity after gelatin substrate SDS-PAGE with conditions optimal for cathepsin renaturing and activity. Densitometric analysis of the zymogram provides quantitative information from this low cost assay. After systematic modifications to optimize cathepsin zymography, we describe reduced electrophoresis time from 2 h to 10 min, incubation assay time from overnight to 4 h, and reduced minimal tissue protein necessary while maintaining sensitive detection limits; an evaluation of the pros and cons of each modification is also included."
According to the news editors, the research concluded: "We further describe image acquisition by Smartphone camera, export to Matlab, and densitometric analysis code to quantify and report cathepsin activity, adding portability and replacing large scale, darkbox imaging equipment that could be cost prohibitive in limited resource settings."
For more information on this research see: Systematic optimization of multiplex zymography protocol to detect active cathepsins K, L, S, and V in healthy and diseased tissue: compromise among limits of detection, reduced time, and resources. Molecular Biotechnology, 2013;54(3):1038-47. Molecular Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Molecular Biotechnology - www.springerlink.com/content/1073-6085/)
The news correspondents report that additional information may be obtained from J.E. Dumas, Wallace H Coulter Dept. of Biomedical Engineering, Georgia Institute of Technology and Emory University, 315 Ferst Dr Suite 1308, Atlanta, GA 30332, United States (see also Biotechnology).
The publisher's contact information for the journal Molecular Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Biotechnology, Atlanta, Georgia, United States, North and Central America.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC