New Findings from Kyoto University in Enzymes and Coenzymes Provides New Insights (Direct analysis of Holliday junction resolving enzyme in a DNA origami nanostructure)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting from Kyoto, Japan, by NewsRx journalists, research stated, "Holliday junction (HJ) resolution is a fundamental step for completion of homologous recombination. HJ resolving enzymes (resolvases) distort the junction structure upon binding and prior cleavage, raising the possibility that the reactivity of the enzyme can be affected by a particular geometry and topology at the junction."
The news correspondents obtained a quote from the research from Kyoto University, "Here, we employed a DNA origami nano-scaffold in which each arm of a HJ was tethered through the base-pair hybridization, allowing us to make the junction core either flexible or inflexible by adjusting the length of the DNA arms. Both flexible and inflexible junctions bound to Bacillus subtilis RecU HJ resolvase, while only the flexible junction was efficiently resolved into two duplexes by this enzyme. This result indicates the importance of the structural malleability of the junction core for the reaction to proceed."
According to the news reporters, the research concluded: "Moreover, cleavage preferences of RecU-mediated reaction were addressed by analyzing morphology of the reaction products."
For more information on this research see: Direct analysis of Holliday junction resolving enzyme in a DNA origami nanostructure. Nucleic Acids Research, 2014;42(11):7421-7428. Nucleic Acids Research can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Nucleic Acids Research - nar.oxfordjournals.org)
Our news journalists report that additional information may be obtained by contacting Y. Suzuki, Kyoto University, Lab Plasma Membrane & Nucl Signaling, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068501, Japan. Additional authors for this research include M. Endo, C. Canas, S. Ayora, J.C. Alonso, H. Sugiyama and K. Takeyasu (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Kyoto, Japan, DNA Research, Enzymes and Coenzymes
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