New Findings from Chinese Academy of Agricultural Sciences in the Area of DNA Vaccines Reported (Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus)
By a News Reporter-Staff News Editor at Vaccine Weekly -- Researchers detail new data in Biotechnology. According to news originating from Changchun, People's Republic of China, by NewsRx correspondents, research stated, "The European (EU) genotype of porcine reproductive and respiratory syndrome virus (Genotype-I PRRSV) has recently emerged in China. The coexistence of Genotype-I and -II PRRSV strains could cause seriously affect PRRSV diagnosis and management."
Our news journalists obtained a quote from the research from the Chinese Academy of Agricultural Sciences, "Current vaccines are not able to protect against PRRSV infection completely and have inherent drawbacks. Thus, genetically engineered vaccines, including DNA vaccine and live vector engineered vaccines, have been developed. This study aimed to determine the enhanced immune responses of mice inoculated with a DNA vaccine coexpressing GP3 and GP5 of a Genotype-I PRRSV. To evaluate the immunogenicity of GP3 and GP5 proteins from European-type PRRSV, three DNA vaccines, pVAX1-EU-ORF3-ORF5, pVAX1-EU-ORF3 and pVAX1-EU-ORF5, were constructed, which were based on a Genotype-I LV strain (GenBank ID: M96262). BALB/c mice were immunized with the DNA vaccines; delivered in the form of chitosan-DNA nanoparticles. To increase the efficiency of the vaccine, Quil A (Quillaja) was used as an adjuvant. GP3 and GP5-specific antibodies, neutralizing antibodies and cytokines (IL-2, IL-4, IL-10 and IFN gamma) from the immunized mice sera, and other immune parameters, were examined, including T-cell proliferation responses and subgroups of spleen T-lymphocytes. The results showed that ORF3 and ORF5 proteins of Genotype-I PRRSV induced GP3 and GP5-specific antibodies that could neutralize the virus. The levels of Cytokines IL-2, IL-4, IL-10, and IFN-gamma. of the experimental groups were significantly higher than those of control groups after booster vaccination (P < 0.05). The production of CD3(+) CD4(+) and CD3(+) CD8(+) T lymphocyte was also induced. T lymphocyte proliferation assays showed that the PRRSV LV strain virus could stimulate the proliferation of T lymphocytes in mice in the experimental group. Using Quil A as adjuvant, Genotype-I PRRSV GP3 and GP5 proteins produced good immunogenicity and reactivity. More importantly, better PRRSV-specific neutralizing antibody titers and cell-mediated immune responses were observed in mice immunized with the DNA vaccine co-expressing GP3 and GP5 proteins than in mice immunized with a DNA vaccine expressing either protein singly."
According to the news editors, the research concluded: "The results of this study demonstrated that co-immunization with GP3 and GP5 produced a better immune response in mice."
For more information on this research see: Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus. BMC Veterinary Research, 2014;10():1-11. BMC Veterinary Research can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; BMC Veterinary Research - www.biomedcentral.com/bmcvetres/)
The news correspondents report that additional information may be obtained from J.Q. Ren, Chinese Academy Agr Sci, Inst Special Anim & Plant Sci, Changchun 130122, People's Republic of China. Additional authors for this research include W.C. Sun, H.J. Lu, S.B. Wen, J. Jing, F.L. Yan, H. Liu, C.X. Liu, P.P. Xiao, X. Chen, S.W. Du, R. Du and N.Y. Jin (see also Biotechnology).
Keywords for this news article include: Asia, Antibodies, Biotechnology, Changchun, Viral DNA, Immunology, Blood Cells, Engineering, RNA Viruses, DNA Research, DNA Vaccines, T-Lymphocytes, Blood Proteins, Swine Diseases, Immunoglobulins, Synthetic Vaccines, Biological Products, Arterivirus Infections, Mononuclear Leukocytes, Nidovirales Infections
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